Cell cycle analysis of 32D/α-ER/FLT3/ITD cells exposed to estradiol
Cell lines and treatment . | G0/G1 phase, % . | S phase, % . | G2/M phase, % . | G0/G1-to-S ratio . |
|---|---|---|---|---|
| 32D/α-ER/FLT3/ITD | ||||
| Before estradiol treatment | 57.7 ± 1.9 | 32.8 ± 0.5 | 8.1 ± 1.9 | 1.8 ± 0.1:1 |
| 24 h estradiol treatment | 72.3 ± 1.2 | 14.9 ± 0.5 | 6.5 ± 0.8 | 4.9 ± 0.2:1 |
| 32D/FLT3/ITD | ||||
| Before estradiol treatment | 48.1 ± 0.4 | 35.2 ± 0.5 | 15.8 ± 0.8 | 1.4 ± 0.02:1 |
| 24 h estradiol treatment | 48.1 ± 0.8 | 34.3 ± 0.5 | 16.4 ± 1.2 | 1.4 ± 0.01:1 |
Cell lines and treatment . | G0/G1 phase, % . | S phase, % . | G2/M phase, % . | G0/G1-to-S ratio . |
|---|---|---|---|---|
| 32D/α-ER/FLT3/ITD | ||||
| Before estradiol treatment | 57.7 ± 1.9 | 32.8 ± 0.5 | 8.1 ± 1.9 | 1.8 ± 0.1:1 |
| 24 h estradiol treatment | 72.3 ± 1.2 | 14.9 ± 0.5 | 6.5 ± 0.8 | 4.9 ± 0.2:1 |
| 32D/FLT3/ITD | ||||
| Before estradiol treatment | 48.1 ± 0.4 | 35.2 ± 0.5 | 15.8 ± 0.8 | 1.4 ± 0.02:1 |
| 24 h estradiol treatment | 48.1 ± 0.8 | 34.3 ± 0.5 | 16.4 ± 1.2 | 1.4 ± 0.01:1 |
Flow cytometric analysis of propidium iodide stained 32Dα-ER/FLT3/ITD and 32D/FLT3/ITD cells was performed prior to and after 24 hours of estradiol exposure. Values are the mean percentage or ratio ± SD from 3 different experiments.