Effect of cytokine stimulation on the engraftment of CD34+ cells in NOD/SCID mice: 48 hours
. | . | CD45+ cells in mouse BM, % . | . | |
|---|---|---|---|---|
| Experiment . | Cells/mouse, 106 . | Fresh . | Postculture . | |
| 1 | 0.5 | 1.4, 1.2 | 0.1 | |
| 1 | 1.0 | 5.8, 16.2 | 0.1, 0.0 | |
| 2 | 1.0 | 1.9, 1.3 | 0.5, 0.1 | |
| Median (range) | NA | 1.7 (1.2-16.0) | 0.1 (0.0-0.5)* | |
| Animals engrafted | NA | 6 of 6 | 0 of 5 | |
. | . | CD45+ cells in mouse BM, % . | . | |
|---|---|---|---|---|
| Experiment . | Cells/mouse, 106 . | Fresh . | Postculture . | |
| 1 | 0.5 | 1.4, 1.2 | 0.1 | |
| 1 | 1.0 | 5.8, 16.2 | 0.1, 0.0 | |
| 2 | 1.0 | 1.9, 1.3 | 0.5, 0.1 | |
| Median (range) | NA | 1.7 (1.2-16.0) | 0.1 (0.0-0.5)* | |
| Animals engrafted | NA | 6 of 6 | 0 of 5 | |
Freshly isolated CD34+ cells (0.5 or 1.0 × 106 cells/animal) or the equal number of cultured cells were infused into sublethally irradiated NOD/SCID mice. Cultured cells were stimulated with SCF (100 ng/mL), FL (100 ng/mL), IL-3 (20 ng/mL), and IL-6 (20 ng/mL) in X-VIVO 10 with 1% FCS for 48 hours. For all experiments, engraftment was assessed 6 weeks after transplantation using immunophenotype analysis of BM cells, as detailed in “Materials and methods.” An animal was considered to have experienced successful engraftment if CD45+ cells comprised 1% or more of BM cells. Data are given for each animal and as the median and range of each group, as indicated.
NA indicates not applicable.
P < .01 compared with fresh cells; Mann-Whitney U test.