Effect of cytokine stimulation on the engraftment of CD34+ cells in NOD/SCID mice: 2 to 5 days
. | Cells/mouse, 106 . | . | CD45+ cells in mouse BM, % . | . | ||
|---|---|---|---|---|---|---|
| Experiment . | Fresh . | Postculture . | Fresh . | Postculture . | ||
| 1 | 0.5 | 0.7 | 2.0, 2.3 | 0.0, 1.3 | ||
| 2 | 0.5 | 2.6 | 4.6 | 2.7 | ||
| 1 | 1.0 | 1.3 | 2.1, 10.5 | 0.1, 0.1 | ||
| 2 | 1.0 | 5.2 | 72.3, 78.4 | 22.7, 0.7 | ||
| Median (range) | NA | NA | 4.6 (2.0-78.4) | 0.7 (0.0-22.7)* | ||
| Animals engrafted | NA | NA | 7 of 7 | 3 of 7 | ||
. | Cells/mouse, 106 . | . | CD45+ cells in mouse BM, % . | . | ||
|---|---|---|---|---|---|---|
| Experiment . | Fresh . | Postculture . | Fresh . | Postculture . | ||
| 1 | 0.5 | 0.7 | 2.0, 2.3 | 0.0, 1.3 | ||
| 2 | 0.5 | 2.6 | 4.6 | 2.7 | ||
| 1 | 1.0 | 1.3 | 2.1, 10.5 | 0.1, 0.1 | ||
| 2 | 1.0 | 5.2 | 72.3, 78.4 | 22.7, 0.7 | ||
| Median (range) | NA | NA | 4.6 (2.0-78.4) | 0.7 (0.0-22.7)* | ||
| Animals engrafted | NA | NA | 7 of 7 | 3 of 7 | ||
Freshly isolated CD34+ cells (0.5 or 1.0 × 106 cells/animal) or the expanded equivalent number of cultured cells were infused into sublethally irradiated NOD/SCID mice. Cultured cells were stimulated with SCF (100 ng/mL), FL (100 ng/mL), IL-3 (20 ng/mL), and IL-6 20 ng/mL) in X-VIVO 10 with 1% FCS for 2 to 5 days. For all experiments, engraftment was assessed 6 weeks after transplantation using immunophenotype analysis of BM cells, as detailed in “Materials and methods.” An animal was considered to have experienced successful engraftment if CD45+ cells comprised 1% or more of BM cells. Data are given for each animal and as the median and range of each group, as indicated.
NA indicates not applicable.
P < .05 compared with fresh cells; Mann-Whitney U test.