Expression of MHC proteins on NK clones
Antibody . | Patient A . | Patient B . | Patient C . | Healthy sister . |
|---|---|---|---|---|
| Secondary F(ab′)2 background | 3.2 ± 0.7 | 2.2 ± 0.3 | 2.8 ± 0.4 | 3.4 ± 0.8 |
| W6/32 F(ab′)2 | 36.7 ± 8.2 | 23 ± 6.5 | 30.7 ± 8.0 | 629 ± 183 |
| BBM-1 F(ab′)2 | 7.7 ± 2.5 | 5.3 ± 1.7 | 7.1 ± 1.6 | 130 ± 30 |
| MHC class II F(ab′)2 | 33.1 ± 3.6 | 54.3 ± 9.6 | 54.6 ± 7.7 | 94.7 ± 9.1 |
Antibody . | Patient A . | Patient B . | Patient C . | Healthy sister . |
|---|---|---|---|---|
| Secondary F(ab′)2 background | 3.2 ± 0.7 | 2.2 ± 0.3 | 2.8 ± 0.4 | 3.4 ± 0.8 |
| W6/32 F(ab′)2 | 36.7 ± 8.2 | 23 ± 6.5 | 30.7 ± 8.0 | 629 ± 183 |
| BBM-1 F(ab′)2 | 7.7 ± 2.5 | 5.3 ± 1.7 | 7.1 ± 1.6 | 130 ± 30 |
| MHC class II F(ab′)2 | 33.1 ± 3.6 | 54.3 ± 9.6 | 54.6 ± 7.7 | 94.7 ± 9.1 |
Forty NK clones were isolated from each of the 3 patients and from the healthy sister. Clones were stained by various mAbs as indicated and analyzed by FACS. All antibodies used were in the form of F(ab′)2 fragments. Background was the secondary mAb in the form of F(ab′)2. Data are presented as the median fluorescence intensity (MFI) of 40 clones ± standard deviation (SD). All 160 clones were stained at the same time.