Summary of erythrocyte lipid raft proteins
Protein . | Molecular weight, kDa . | Membrane association* . | Remarks . | Internalized to the PVM? . |
|---|---|---|---|---|
| α-globin | 15 | Cytoplasmic | Hemoglobin complex | N/D |
| β-globin | 16 | Cytoplasmic | Hemoglobin complex | N/D |
| GAPDH | 36 | Cytoplasmic | Conversion of G3P to 1,3-BPG | Not internalized |
| Peroxiredoxin-2 | 21.9 | Cytoplasmic | Eliminates peroxides; signaling? | N/D |
| S-100β | 10.5 | Cytoplasmic | Dimer with α chain; binds p53, tubulin, and Ca2+; (dis)assembly of microtubules and intermediate filaments | N/D |
| CA-I | 28.8 | Cytoplasmic | Reversible hydration of CO2 | N/D |
| Flotillin-1 | 47 | Endofacing hairpin loop | Organization of caveolae and/or lipid rafts | Internalized |
| Flotillin-2 | 45 | Endofacing hairpin loop | High-order flotillin oligomers; raft scaffolding component | Internalized |
| Stomatin | 31 | Endofacing hairpin loop | Associates with Glut1; cation transport | Not internalized |
| Gαs | 44 | Endofacing | GPCR activation of adenylate cyclase | Internalized |
| CD55 | 55-70 | GPI-linked | Decay accelerating factor | Internalized |
| CD58 | 64-73 | GPI-linked | Unknown function in erythrocytes | Internalized |
| CD59 | 20-40 | GPI-linked | Membrane inhibitor of reactive complement lysis | Internalized |
| Glut1 | 54 | Multipass (12) | May bind stomatin; passive glucose transport | Not internalized |
| Band 3 | 101 | Multipass (14) | Binds protein 4.2 and ankyrin; “Cl shift” | Not internalized |
| AQP1 | 28 | Multipass (6) | Water channel protein for erythrocytes and renal PCT | Internalized |
| β2-AR | 65 | Multipass (7) | Gαs-coupled receptor | Internalized |
| Duffy | 35-43 | Multipass (7) | Chemokine and P vivax receptor; GPCR-like | Internalized |
| Scramblase | 35 | Single-pass | Movement of membrane phospholipids | Internalized |
Protein . | Molecular weight, kDa . | Membrane association* . | Remarks . | Internalized to the PVM? . |
|---|---|---|---|---|
| α-globin | 15 | Cytoplasmic | Hemoglobin complex | N/D |
| β-globin | 16 | Cytoplasmic | Hemoglobin complex | N/D |
| GAPDH | 36 | Cytoplasmic | Conversion of G3P to 1,3-BPG | Not internalized |
| Peroxiredoxin-2 | 21.9 | Cytoplasmic | Eliminates peroxides; signaling? | N/D |
| S-100β | 10.5 | Cytoplasmic | Dimer with α chain; binds p53, tubulin, and Ca2+; (dis)assembly of microtubules and intermediate filaments | N/D |
| CA-I | 28.8 | Cytoplasmic | Reversible hydration of CO2 | N/D |
| Flotillin-1 | 47 | Endofacing hairpin loop | Organization of caveolae and/or lipid rafts | Internalized |
| Flotillin-2 | 45 | Endofacing hairpin loop | High-order flotillin oligomers; raft scaffolding component | Internalized |
| Stomatin | 31 | Endofacing hairpin loop | Associates with Glut1; cation transport | Not internalized |
| Gαs | 44 | Endofacing | GPCR activation of adenylate cyclase | Internalized |
| CD55 | 55-70 | GPI-linked | Decay accelerating factor | Internalized |
| CD58 | 64-73 | GPI-linked | Unknown function in erythrocytes | Internalized |
| CD59 | 20-40 | GPI-linked | Membrane inhibitor of reactive complement lysis | Internalized |
| Glut1 | 54 | Multipass (12) | May bind stomatin; passive glucose transport | Not internalized |
| Band 3 | 101 | Multipass (14) | Binds protein 4.2 and ankyrin; “Cl shift” | Not internalized |
| AQP1 | 28 | Multipass (6) | Water channel protein for erythrocytes and renal PCT | Internalized |
| β2-AR | 65 | Multipass (7) | Gαs-coupled receptor | Internalized |
| Duffy | 35-43 | Multipass (7) | Chemokine and P vivax receptor; GPCR-like | Internalized |
| Scramblase | 35 | Single-pass | Movement of membrane phospholipids | Internalized |
Nineteen proteins were reliably identified in the floating fraction of Tx-100-resistant erythrocyte membranes in this and other studies,1,3,12 but only a subset of these proteins enters the malarial vacuole.
N/D indicates no data; G3P, glyceraldehyde-3-phosphate; 1,3-BPG, 1-3,bisphosphoglycerate; GPCR, G protein—coupled receptor; PCT, proximal convoluted tubule.
Numbers in parentheses represent the number of transmembrane domains in multipass proteins.