Erythrocyte raft proteins identified by LC-MS/MS
Gel band . | Protein . | No. of peptides . |
|---|---|---|
| High-confidence assignments* | ||
| 250 | Band 3 | 14 |
| 120 | Band 3 | 21 |
| 50 | Flotillin-1 | 16 |
| 50 | Flotillin-2 | 11 |
| 50 | Glucose transporter 1 | 3 |
| 45 | Peroxiredoxin-2 | 7 |
| 45 | Band 3 | 6 |
| 32 | Stomatin | 13 |
| 30 | Stomatin | 5 |
| 30 | Carbonic anhydrase I | 4 |
| 28 | Flotillin-1 | 4 |
| 28 | Stomatin | 4 |
| 28 | S100β | 3 |
| 28 | Peroxiredoxin-2 | 3 |
| 15 | α-globin | 6 |
| 15 | β-globin | 14 |
| Tentative assignments* | ||
| 250 | Glucose transporter 1 | 2 |
| 120 | Protein 4.2 (pallidin) | 1 |
| 120 | S100β | 1 |
| 120 | Peroxiredoxin-2 | 1 |
| 50 | S100β | 1 |
| 50 | Peroxiredoxin-2 | 1 |
| 45 | GAPDH | 2 |
| 45 | Stomatin | 1 |
| 32 | S100β | 2 |
| 30 | Carbonic anhydrase II | 2 |
| 30 | S100β | 1 |
| 30 | Peroxiredoxin-2 | 1 |
| 28 | Glucose transporter 1 | 2 |
| 28 | Flotillin-2 | 2 |
| 28 | Aquaporin-1 | 1 |
Gel band . | Protein . | No. of peptides . |
|---|---|---|
| High-confidence assignments* | ||
| 250 | Band 3 | 14 |
| 120 | Band 3 | 21 |
| 50 | Flotillin-1 | 16 |
| 50 | Flotillin-2 | 11 |
| 50 | Glucose transporter 1 | 3 |
| 45 | Peroxiredoxin-2 | 7 |
| 45 | Band 3 | 6 |
| 32 | Stomatin | 13 |
| 30 | Stomatin | 5 |
| 30 | Carbonic anhydrase I | 4 |
| 28 | Flotillin-1 | 4 |
| 28 | Stomatin | 4 |
| 28 | S100β | 3 |
| 28 | Peroxiredoxin-2 | 3 |
| 15 | α-globin | 6 |
| 15 | β-globin | 14 |
| Tentative assignments* | ||
| 250 | Glucose transporter 1 | 2 |
| 120 | Protein 4.2 (pallidin) | 1 |
| 120 | S100β | 1 |
| 120 | Peroxiredoxin-2 | 1 |
| 50 | S100β | 1 |
| 50 | Peroxiredoxin-2 | 1 |
| 45 | GAPDH | 2 |
| 45 | Stomatin | 1 |
| 32 | S100β | 2 |
| 30 | Carbonic anhydrase II | 2 |
| 30 | S100β | 1 |
| 30 | Peroxiredoxin-2 | 1 |
| 28 | Glucose transporter 1 | 2 |
| 28 | Flotillin-2 | 2 |
| 28 | Aquaporin-1 | 1 |
In addition to the reported protein identifications, β-globin and sometimes α-globin were detected in all bands analyzed. For simplicity, these results were omitted from the table.
Protein identifications were considered high-confidence assignments if the TurboSEQUEST browser report matched 3 or more tryptic peptides with a cross-correlation score of 2.5 or higher. Identifications were considered tentative if only 1 or 2 peptides were matched with cross-correlation scores of 2.5 or higher. The number of peptides meeting these criteria is shown.