Amino acid sequence of TCRα and β-chain junction of BHRF1-specific CD4 T-cell clones
. | . | TCR α-chain sequence . | . | . | TCR β-chain sequence . | . | . | No. of clones . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor . | % Vβ . | Vα . | CDR3 alpha . | Jα . | Vβ . | CDR3 . | Jβ . | . | ||||
| D3 | 71% Vb17 | Vα6s1 | CAM REIQGAQKL VFG | Jα54 | Vβ17s1 | CAS SPADGTMNTEA FFG | Jβ1s1 | 2/2 (LP 15) | ||||
| 25% Vb3 | Vα6s1 | CAM RGITGANSKL TFG | Jα56 | Vβ3s1 | CAS TLQTGAMNTEA FFG | Jβ1s1 | 2/2 (LP45) | |||||
| RA1 | 8% Vb9 | Vα14s1 | CAY RRTGANSKL TFG | Jα56 | Vβ13s1 | CAS SPGTGTYTEA FFG | Jβ1s1 | 2/2 (A22) | ||||
| ? | Vα1s4 | CAV VLRTGANNL FFG | Jα36 | |||||||||
| RA5 | 5% Vb3 | Vα6s1 | CAM REIYNFNKF YFG | Jα21 | Vβ3s1 | CAS SQSTGTMNTEA FFG | Jβ1s1 | 2/2 | ||||
| 30% Vb17 | ||||||||||||
| 16% Vb20 | ||||||||||||
| RA14 | ? | Vα16s1 | CAH RRDTGFQKL VFG | Jα8 | Vβ3s1 | CAS SLSRGDWGSPL HFG | Jβ1s6 | 2/2 | ||||
| RA3 | 100% Vb9 | Vα23s1 | CAV VNAGGTSYGKL TFG | Jα52 | Vβ9s1 | CAS SLRQGGTEA FFG | Jβ1s1 | 2/2 | ||||
. | . | TCR α-chain sequence . | . | . | TCR β-chain sequence . | . | . | No. of clones . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor . | % Vβ . | Vα . | CDR3 alpha . | Jα . | Vβ . | CDR3 . | Jβ . | . | ||||
| D3 | 71% Vb17 | Vα6s1 | CAM REIQGAQKL VFG | Jα54 | Vβ17s1 | CAS SPADGTMNTEA FFG | Jβ1s1 | 2/2 (LP 15) | ||||
| 25% Vb3 | Vα6s1 | CAM RGITGANSKL TFG | Jα56 | Vβ3s1 | CAS TLQTGAMNTEA FFG | Jβ1s1 | 2/2 (LP45) | |||||
| RA1 | 8% Vb9 | Vα14s1 | CAY RRTGANSKL TFG | Jα56 | Vβ13s1 | CAS SPGTGTYTEA FFG | Jβ1s1 | 2/2 (A22) | ||||
| ? | Vα1s4 | CAV VLRTGANNL FFG | Jα36 | |||||||||
| RA5 | 5% Vb3 | Vα6s1 | CAM REIYNFNKF YFG | Jα21 | Vβ3s1 | CAS SQSTGTMNTEA FFG | Jβ1s1 | 2/2 | ||||
| 30% Vb17 | ||||||||||||
| 16% Vb20 | ||||||||||||
| RA14 | ? | Vα16s1 | CAH RRDTGFQKL VFG | Jα8 | Vβ3s1 | CAS SLSRGDWGSPL HFG | Jβ1s6 | 2/2 | ||||
| RA3 | 100% Vb9 | Vα23s1 | CAV VNAGGTSYGKL TFG | Jα52 | Vβ9s1 | CAS SLRQGGTEA FFG | Jβ1s1 | 2/2 | ||||
BHRF1-specific CD4 T-cell clones were derived from BHRF1-specific T-cell lines sorted with BHRF1/DR*0401 multimers.