Quantitative analysis of engraftment after lineage-depleted, double cord transplantation
No. experiments . | HLA disparity . | Donor A, % STR* . | Donor B, % STR*x . | Donor cell ratio . | Double-single engraft ratio . | Lineages of donor cells, % . |
|---|---|---|---|---|---|---|
| 2 | 4 or 5 mismatch | 67.5 ± 4.8 | 32.5 ± 4.8 | 2.1:1 | 2.0 | Lymph, 65.7 ± 3.7; Myelo, 3.4 ± 1.1; Erythro, 11.7 ± 5.3 |
No. experiments . | HLA disparity . | Donor A, % STR* . | Donor B, % STR*x . | Donor cell ratio . | Double-single engraft ratio . | Lineages of donor cells, % . |
|---|---|---|---|---|---|---|
| 2 | 4 or 5 mismatch | 67.5 ± 4.8 | 32.5 ± 4.8 | 2.1:1 | 2.0 | Lymph, 65.7 ± 3.7; Myelo, 3.4 ± 1.1; Erythro, 11.7 ± 5.3 |
Two additional cohorts of double cord transplantations were analyzed for donor distribution of engrafted cells by RQ-STR, and the relative ratio of engraftment levels of double- over single-unit transplantation (double-single engraft ratio) was calculated. Lineages of donor cells were determined by percentages of lymphoid (Lymph; CD19/20), myeloid (Myelo; CD15/66b), and erythroid (Erythro; glycophorin-A) cells among the total human cells engrafted (CD45/71) (n = 11 for single; n = 10 for double transplantations).
Values presented as mean ± SEM.