Cumulative quantitation of donor cell distribution using RQ-STR analysis in double cord transplantation of total MNCs
HLA disparity . | No. experiments (no. mice) . | Donor A, % STR* . | Donor B, % STR* . | Donor cell ratio . | No. mice with predominance . |
|---|---|---|---|---|---|
| Full match | 3 (5) | 82.8 ± 7.1 | 17.2 ± 7.1 | 4.8:1 | 4 of 5 |
| 1 mismatch | 2 (8) | 83.8 ± 2.2 | 16.2 ± 2.2 | 5.2:1 | 8 of 8 |
| 2 mismatches | 2 (5) | 74.3 ± 5.6 | 25.7 ± 5.6 | 2.9:1 | 3 of 5 |
| Full mismatch | 2 (4) | 80.6 ± 3.5 | 19.4 ± 3.5 | 4.2:1 | 3 of 4 |
| Total | 9 (22) | 80.7 ± 2.2 | 19.3 ± 2.2 | 4.2:1 | 18 of 22 |
HLA disparity . | No. experiments (no. mice) . | Donor A, % STR* . | Donor B, % STR* . | Donor cell ratio . | No. mice with predominance . |
|---|---|---|---|---|---|
| Full match | 3 (5) | 82.8 ± 7.1 | 17.2 ± 7.1 | 4.8:1 | 4 of 5 |
| 1 mismatch | 2 (8) | 83.8 ± 2.2 | 16.2 ± 2.2 | 5.2:1 | 8 of 8 |
| 2 mismatches | 2 (5) | 74.3 ± 5.6 | 25.7 ± 5.6 | 2.9:1 | 3 of 5 |
| Full mismatch | 2 (4) | 80.6 ± 3.5 | 19.4 ± 3.5 | 4.2:1 | 3 of 4 |
| Total | 9 (22) | 80.7 ± 2.2 | 19.3 ± 2.2 | 4.2:1 | 18 of 22 |
Total MNCs equivalent to 5 × 104 CD34+ cells from each UCB unit were mixed and transplanted. Percentages of donor A (dominant party) and donor B (nondominant party) were determined by measuring the area of donor-specific STR peaks among all human-specific STR peaks, and the donor A-to-donor B cell ratio was calculated. Ratios greater than 3:1 were considered dominant, and the mice were scored as donor predominant. HLA disparity refers to HLA-A, HLA-B, and HLA-DR.
Values presented as mean ± SEM.