Yeast transformation assay of EBP with EEN family members
DNA-BD . | AD . | β-Galactosidase assay . |
|---|---|---|
| — | Clone no. 30 | - |
| EEN | — | - |
| EEN | Clone no. 30 | + |
| B1 | Clone no. 30 | - |
| B2 | Clone no. 30 | + |
DNA-BD . | AD . | β-Galactosidase assay . |
|---|---|---|
| — | Clone no. 30 | - |
| EEN | — | - |
| EEN | Clone no. 30 | + |
| B1 | Clone no. 30 | - |
| B2 | Clone no. 30 | + |
Clone no. 30 in Gal4-AD plasmid was cotransformed with each EEN family member, EEN, B1, and B2, cloned in Gal4-BD plasmid into yeast strain SFY526. Colony lift filter assay was then performed on the cotransformants. Positive interaction is indicated by +, and no interaction is represented by -.
— indicates no proteins.