Affinities of peptides for low-Mr heparin
Peptide . | Kd, nM . |
|---|---|
| (ARKKAAKA)4 | 55 ± 32 |
| Cys-(ARKKAAKA)4-Cys-Cyclized | 38 ± 13 |
| (ARKKAAKA)3ARKKCAKA | 54 ± 34 |
| (ARKKAAKA)3VLVLVLVL | 57 ± 23 |
| (ARKKAAKA)3VLVLVL | 44 ± 17 |
| (AKKARA)6 | 58 ± 16 |
| VLVL(ARKKAAKA)3ARKKCAKA | 48 ± 11 |
| ARKKAAKA(A16)ARKKAAKA | 8300 ± 60 |
| VLVLARKKAAKAPARKKAAKAVLVL | 5200 ± 1548 |
Peptide . | Kd, nM . |
|---|---|
| (ARKKAAKA)4 | 55 ± 32 |
| Cys-(ARKKAAKA)4-Cys-Cyclized | 38 ± 13 |
| (ARKKAAKA)3ARKKCAKA | 54 ± 34 |
| (ARKKAAKA)3VLVLVLVL | 57 ± 23 |
| (ARKKAAKA)3VLVLVL | 44 ± 17 |
| (AKKARA)6 | 58 ± 16 |
| VLVL(ARKKAAKA)3ARKKCAKA | 48 ± 11 |
| ARKKAAKA(A16)ARKKAAKA | 8300 ± 60 |
| VLVLARKKAAKAPARKKAAKAVLVL | 5200 ± 1548 |
Binding affinity constants were determined by ACE as described in the text. Each number represents the mean and standard deviation of 4 independent assays. The monomer ARKKAAKA had negligible affinity.
Representative Phosphorimages are shown in Figure 1.