Maintenance of short- and long-term FL SRCs in culture
FL specimen and mouse no. . | Engraftment, % . |
|---|---|
| 6 wk after transplantation | |
| FL no. 813 | |
| 204 | 8.8 |
| 211 | 18.8 |
| FL no. 852 | |
| 200 | 2.4 |
| 213 | 7.9 |
| FL no. 841 | |
| 259 | 2.0 |
| 260 | 9.1 |
| 12 wk after transplantation | |
| FL no. 813 | |
| 206 | 8.3 |
| 205 | 24.2 |
| 207 | 33.5 |
| FL no. 852 | |
| 201 | 4.1 |
| 202 | 11.8 |
| 203 | 18.7 |
| FL no. 841 | |
| 242 | 22.8 |
| 243 | 7.6 |
| 244 | 3.8 |
FL specimen and mouse no. . | Engraftment, % . |
|---|---|
| 6 wk after transplantation | |
| FL no. 813 | |
| 204 | 8.8 |
| 211 | 18.8 |
| FL no. 852 | |
| 200 | 2.4 |
| 213 | 7.9 |
| FL no. 841 | |
| 259 | 2.0 |
| 260 | 9.1 |
| 12 wk after transplantation | |
| FL no. 813 | |
| 206 | 8.3 |
| 205 | 24.2 |
| 207 | 33.5 |
| FL no. 852 | |
| 201 | 4.1 |
| 202 | 11.8 |
| 203 | 18.7 |
| FL no. 841 | |
| 242 | 22.8 |
| 243 | 7.6 |
| 244 | 3.8 |
Three FL samples were expanded for 28 days, and 20 × 106 viable nucleated cells were injected into individual NOD/SCID mice. Engraftment was analyzed about 6 weeks (short-term) or about 12 weeks (long-term) after transplantation and is expressed as the percentage of CD45+ human cells in the mouse BM.