Table 2.

Representative data for expression of CD18 integrins determined by immunofluorescence flow cytometry of PMN and lymphocyte subsets (CD3+, CD4+, CD8+) in whole blood of the patient and a healthy adult control


Antigen

Source*

PMNs

PMN/fMLF

CD3+

CD4+

CD8+
CD18   C   128  572   1632   1262   1953  
CD18   P   41   131   236   300   437  
CD11a   C   63   75   760   542   890  
CD11a   P   32   41   110   137   127  
CD11b   C   314   2115   —   —   —  
CD11b
 
P
 
54
 
510
 

 

 

 

Antigen

Source*

PMNs

PMN/fMLF

CD3+

CD4+

CD8+
CD18   C   128  572   1632   1262   1953  
CD18   P   41   131   236   300   437  
CD11a   C   63   75   760   542   890  
CD11a   P   32   41   110   137   127  
CD11b   C   314   2115   —   —   —  
CD11b
 
P
 
54
 
510
 

 

 

 

—indicates marker not assayed.

*

Blood from the adult control (C) or the patient (P).

fMLF (10 nM) was added to stimulate translocation of stored CD11b/CD18 to the PMN surface.

Value for mean fluorescence intensity, using directly conjugated mAbs to each subunit antigen.

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