Induction by PMN lysates of cytotoxicity of Jurkat cells
Induction treatment . | Cells excluded PI, % n = 5 . | Cell death by trypan blue exclusion, % n = 3 . |
|---|---|---|
| Jurkat cells treated with PMN lysates* | ||
| 2 h | 5.5-20.5‡ | 5-15 |
| 4 h | 11-39.9 | 7-21 |
| Jurkat cells treated with PMN lysates adsorbed to:† | ||
| IgG | 32/25.7 | 22.5 ± 4 |
| Antiperforin | 12.7/15.3 | 12.0 ± 4 |
| Anti-granzyme B | 12.2/6.8 | 16.0 ± 3 |
| Not adsorbed | ND/43.4 | ND |
Induction treatment . | Cells excluded PI, % n = 5 . | Cell death by trypan blue exclusion, % n = 3 . |
|---|---|---|
| Jurkat cells treated with PMN lysates* | ||
| 2 h | 5.5-20.5‡ | 5-15 |
| 4 h | 11-39.9 | 7-21 |
| Jurkat cells treated with PMN lysates adsorbed to:† | ||
| IgG | 32/25.7 | 22.5 ± 4 |
| Antiperforin | 12.7/15.3 | 12.0 ± 4 |
| Anti-granzyme B | 12.2/6.8 | 16.0 ± 3 |
| Not adsorbed | ND/43.4 | ND |
ND indicates not done.
Lysates obtained by repeated freeze-thawing cycles of PMNs (1 × 107) of individual donors as indicated.
Two independent experiments with lysates obtained from PMNs of different donors. Killing was measured after 2 hours. Results of the 2 experiments are separated by the virgule (experiment 1/experiment 2).
All values are corrected for nonspecific killing.