PCR primers used in site-directed mutagenesis of human D1D2
K59Efor | CCAGCCAGACTTCTCTCCATGGCCGGGATG |
| K59Eback | CATCCCGGCCATGGAGAGAAGTCTGGCTGG |
| R60Aback | CCCGGCCATGAAGGCTAGCCTGGCTGGACGCTACC |
| R60Afor | GGTAGCGTCCAGCCAGGCTAGCCTTCATGGCCGGG |
| F91Aback | GGAGCTCGTTGCCACCGGTGTTGCTGCCAAACCCTCG |
| F91Afor | CGAGGGTTTGGCAGCAACACCGGTGGCAACGAGCTCC |
| R117Pback | GGTCAAAGCCATACGGAGTCTGACACTGTAGGG |
| R117Pfor | CCCTACAGTGTCAGACTCCGTATGGCTTTGACC |
| Y118Aback | CCTACAGTGTCAGACTCGGGCCGGCTTTGACCAATTTGC |
| Y118Afor | GCAAATTGGTCAAAGCCGGCCCGAGTCTGACACTGTAGG |
| F120Aback | GTGTCAGACTCGGTATGGCGCCGACCAATTTGCTCTG |
| F120Afor | CAGAGCAAATTGGTCGGCGCCATACCGAGTCTGACAC |
| R139Aback | GCCCTACAAGAATCCCGAAGCTTGGTACCGGGCTAGTTTCCC |
| R139Afor | GGGAAACTAGCCCGGTACCAAGCTTCGGGATTCTTGTAGGGC |
| S164Aback | CGATGCTACAGCTTCGCTAGCAGGGACCCATACC |
| S164Afor | GGTATGGGTCCCTGCTAGCGAAGCTGTAGCATCG |
| R166Sfor | CGACCACAGGTATGGGTCGCTGCTCGAGAAGC |
| R166Sback | GCTTCTCGAGCAGCGACCCATACCTGTGGTCG |
K59Efor | CCAGCCAGACTTCTCTCCATGGCCGGGATG |
| K59Eback | CATCCCGGCCATGGAGAGAAGTCTGGCTGG |
| R60Aback | CCCGGCCATGAAGGCTAGCCTGGCTGGACGCTACC |
| R60Afor | GGTAGCGTCCAGCCAGGCTAGCCTTCATGGCCGGG |
| F91Aback | GGAGCTCGTTGCCACCGGTGTTGCTGCCAAACCCTCG |
| F91Afor | CGAGGGTTTGGCAGCAACACCGGTGGCAACGAGCTCC |
| R117Pback | GGTCAAAGCCATACGGAGTCTGACACTGTAGGG |
| R117Pfor | CCCTACAGTGTCAGACTCCGTATGGCTTTGACC |
| Y118Aback | CCTACAGTGTCAGACTCGGGCCGGCTTTGACCAATTTGC |
| Y118Afor | GCAAATTGGTCAAAGCCGGCCCGAGTCTGACACTGTAGG |
| F120Aback | GTGTCAGACTCGGTATGGCGCCGACCAATTTGCTCTG |
| F120Afor | CAGAGCAAATTGGTCGGCGCCATACCGAGTCTGACAC |
| R139Aback | GCCCTACAAGAATCCCGAAGCTTGGTACCGGGCTAGTTTCCC |
| R139Afor | GGGAAACTAGCCCGGTACCAAGCTTCGGGATTCTTGTAGGGC |
| S164Aback | CGATGCTACAGCTTCGCTAGCAGGGACCCATACC |
| S164Afor | GGTATGGGTCCCTGCTAGCGAAGCTGTAGCATCG |
| R166Sfor | CGACCACAGGTATGGGTCGCTGCTCGAGAAGC |
| R166Sback | GCTTCTCGAGCAGCGACCCATACCTGTGGTCG |
Back primers amplify the antisense strand and for(ward) primers amplify the sense strand of pCR 2.1-hD1D2. Underlined bases correspond to restriction sites introduced by the primers.