Table 1. PCR primers used in... | American Society of Hematology

Table 1.

PCR primers used in site-directed mutagenesis of human D1D2

K59Efor	CCAGCCAGACTTCTCTCCATGGCCGGGATG
K59Eback	CATCCCGGCCATGGAGAGAAGTCTGGCTGG
R60Aback	CCCGGCCATGAAGGCTAGCCTGGCTGGACGCTACC
R60Afor	GGTAGCGTCCAGCCAGGCTAGCCTTCATGGCCGGG
F91Aback	GGAGCTCGTTGCCACCGGTGTTGCTGCCAAACCCTCG
F91Afor	CGAGGGTTTGGCAGCAACACCGGTGGCAACGAGCTCC
R117Pback	GGTCAAAGCCATACGGAGTCTGACACTGTAGGG
R117Pfor	CCCTACAGTGTCAGACTCCGTATGGCTTTGACC
Y118Aback	CCTACAGTGTCAGACTCGGGCCGGCTTTGACCAATTTGC
Y118Afor	GCAAATTGGTCAAAGCCGGCCCGAGTCTGACACTGTAGG
F120Aback	GTGTCAGACTCGGTATGGCGCCGACCAATTTGCTCTG
F120Afor	CAGAGCAAATTGGTCGGCGCCATACCGAGTCTGACAC
R139Aback	GCCCTACAAGAATCCCGAAGCTTGGTACCGGGCTAGTTTCCC
R139Afor	GGGAAACTAGCCCGGTACCAAGCTTCGGGATTCTTGTAGGGC
S164Aback	CGATGCTACAGCTTCGCTAGCAGGGACCCATACC
S164Afor	GGTATGGGTCCCTGCTAGCGAAGCTGTAGCATCG
R166Sfor	CGACCACAGGTATGGGTCGCTGCTCGAGAAGC
R166Sback	GCTTCTCGAGCAGCGACCCATACCTGTGGTCG

Back primers amplify the antisense strand and for(ward) primers amplify the sense strand of pCR 2.1-hD1D2. Underlined bases correspond to restriction sites introduced by the primers.

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