Correlation between HLA-II phenotype and susceptibility to HTLV-2 infection in human B- and T-cell lines
. | HLA-II phenotype* . | . | tax2b†day 2 a.i. . | tax2b†day 15 a.i. . | p 19 Ag‡day 15 a.i. . | % p 19 Ag IIF positive cells§day 15 a.i. . | CIITA expression . | |
|---|---|---|---|---|---|---|---|---|
| Cell line . | FITC . | DR . | . | . | . | . | . | |
| Raji | 0.5 | 21.4 | 420 | 748 | 6 | 0.3 | + | |
| RJ2.2.5 | 0.4 | 0.5 | 512 | 22 870 | 128 | > 30 | − | |
| RJ/CIITA | 0.4 | 22.5 | 720 | 225 | neg | ND | + | |
| MOLT-4 | 0.3 | 0.3 | 683 | 4 445 | 42 | 3 | − | |
| M4/CIITA | 0.4 | 24.5 | 461 | 213 | neg | ND | + | |
| BLS-2 | 0.4 | 0.6 | 446 | 3 466 | 26 | 2 | − | |
| BLS-1 | 0.7 | 0.7 | 210 | 50 | neg | ND | + | |
. | HLA-II phenotype* . | . | tax2b†day 2 a.i. . | tax2b†day 15 a.i. . | p 19 Ag‡day 15 a.i. . | % p 19 Ag IIF positive cells§day 15 a.i. . | CIITA expression . | |
|---|---|---|---|---|---|---|---|---|
| Cell line . | FITC . | DR . | . | . | . | . | . | |
| Raji | 0.5 | 21.4 | 420 | 748 | 6 | 0.3 | + | |
| RJ2.2.5 | 0.4 | 0.5 | 512 | 22 870 | 128 | > 30 | − | |
| RJ/CIITA | 0.4 | 22.5 | 720 | 225 | neg | ND | + | |
| MOLT-4 | 0.3 | 0.3 | 683 | 4 445 | 42 | 3 | − | |
| M4/CIITA | 0.4 | 24.5 | 461 | 213 | neg | ND | + | |
| BLS-2 | 0.4 | 0.6 | 446 | 3 466 | 26 | 2 | − | |
| BLS-1 | 0.7 | 0.7 | 210 | 50 | neg | ND | + | |
ND indicates not detected; and neg, negative.
Analysis was performed by indirect immunofluorescence and flow cytometry. Values are expressed as mean fluorescence of HLA-II-positive population assessed by an anti-DR mAb (DR) as compared to the corresponding negative control assessed by an isotype-matched irrelevant monoclonal antibody (mAb) (fluorescein isothiocynate [FITC]).
Values express the HTLV-2 proviral load in the cells, as assessed by Taqman real-time PCR of tax2b sequence present per 105 cells of established cultures at 2 and 15 days after infection (a.i.) and normalized versus Albumin gene.
Values express the amount (pg/mL) of HTLV-2 p 19 antigen detected in the supernatant of infected cells by an enzyme immunoassay.
Analysis was performed by indirect immunofluorescence and confocal microscopy. Values refer to the percentage of positive cells.