Contaminating fibroblasts and CMV copies in whole blood and pooled platelet units before and after LD (n = 6)
. | A . | B . | C . | D . | E . | F . | Mean (SD) . | Mean copy no. . |
|---|---|---|---|---|---|---|---|---|
| Whole blood | ||||||||
| Fibroblasts per microliter | ||||||||
| Before LD | 2.60 | 1.30 | 4.37 | 2.63 | 9.47 | 2.83 | 3.87 (2.91)* | — |
| After LD | 0.00 | 0.00 | 0.00 | 0.15 | 0.00 | 0.00 | 0.03* | — |
| Corrected CMV copies per microliter | ||||||||
| Before LD | 4060 | 188 | 420 | 2880 | 1760 | 103 | 2.87 (0.66)† | 742 |
| After LD | 3.96 | 0.51 | 1.07 | 3.77 | 0.85 | 0.30 | 0.053 (0.45)† | 1.13 |
| Log10 CMV reduction | 3.00 | 2.56 | 2.59 | 2.88 | 3.31 | 2.53 | 2.81 (0.31)‡ | — |
| Platelets | ||||||||
| Fibroblasts per microliter | ||||||||
| Before LD | 8.2 | 4.4 | 3.3 | 4.2 | 8.1 | 6.1 | 5.72 (2.09)* | — |
| After LD | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00* | — |
| Corrected CMV copies per microliter | ||||||||
| Before LD | 88.4 | 631 | 824 | 1960 | 57.9 | 598 | 2.58 (0.59)† | 380 |
| After LD | 0.44 | 2.06 | 2.19 | 77.9 | 4.26 | 17.9 | 0.679 (0.79)† | 4.77 |
| Log10 CMV reduction | 2.30 | 2.49 | 2.57 | 1.40 | 1.13 | 1.52 | 1.90 (0.64)‡ | — |
. | A . | B . | C . | D . | E . | F . | Mean (SD) . | Mean copy no. . |
|---|---|---|---|---|---|---|---|---|
| Whole blood | ||||||||
| Fibroblasts per microliter | ||||||||
| Before LD | 2.60 | 1.30 | 4.37 | 2.63 | 9.47 | 2.83 | 3.87 (2.91)* | — |
| After LD | 0.00 | 0.00 | 0.00 | 0.15 | 0.00 | 0.00 | 0.03* | — |
| Corrected CMV copies per microliter | ||||||||
| Before LD | 4060 | 188 | 420 | 2880 | 1760 | 103 | 2.87 (0.66)† | 742 |
| After LD | 3.96 | 0.51 | 1.07 | 3.77 | 0.85 | 0.30 | 0.053 (0.45)† | 1.13 |
| Log10 CMV reduction | 3.00 | 2.56 | 2.59 | 2.88 | 3.31 | 2.53 | 2.81 (0.31)‡ | — |
| Platelets | ||||||||
| Fibroblasts per microliter | ||||||||
| Before LD | 8.2 | 4.4 | 3.3 | 4.2 | 8.1 | 6.1 | 5.72 (2.09)* | — |
| After LD | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00* | — |
| Corrected CMV copies per microliter | ||||||||
| Before LD | 88.4 | 631 | 824 | 1960 | 57.9 | 598 | 2.58 (0.59)† | 380 |
| After LD | 0.44 | 2.06 | 2.19 | 77.9 | 4.26 | 17.9 | 0.679 (0.79)† | 4.77 |
| Log10 CMV reduction | 2.30 | 2.49 | 2.57 | 1.40 | 1.13 | 1.52 | 1.90 (0.64)‡ | — |
The number of CMV copies is corrected for fibroblast contamination at 3 CMV copies per fibroblast.—indicates not applicable.
* Mean cell number (SD); †mean log copy number (SD); ‡mean log reduction (SD).