CMV recovery in the mononuclear cell preparation used to spike whole blood units (n = 6) and platelet units (n = 6)
CMV copies . | A . | B . | C . | D . | E . | F . | Mean log copy no. (SD) . | Mean copy no. . |
|---|---|---|---|---|---|---|---|---|
| Spiking samples for whole blood units | ||||||||
| After coculture | 1.64 × 109 | 2.52 × 108 | 1.42 × 109 | 5.92 × 108 | 1.53 × 109 | 2.75 × 108 | 8.86 (0.38) | 7.26 × 108 |
| After purification, in PBMCs for spiking | 5.08 × 107 | 4.69 × 106 | 2.90 × 107 | 3.70 × 107 | 2.50 × 107 | 3.19 × 106 | 7.22 (0.50) | 1.65 × 107 |
| % CMV recovery* | 3.1 | 1.86 | 2.04 | 6.25 | 1.63 | 1.16 | — | — |
| CMV signal in final spike attributable to fibroblasts | 3.3 × 105 | 7.5 × 105 | 9.9 × 103 | 8.1 × 103 | 8.1 × 102 | 1.2 × 104 | 4.38 (1.11) | 2.4 × 104 |
| % CMV from fibroblasts† | 0.65 | 16 | 0.03 | 0.02 | 0.003 | 0.37 | — | — |
| Spiking samples for platelet units | ||||||||
| After coculture | 1.8 × 108 | 5.1 × 108 | 1.5 × 109 | 1.8 × 109 | 4.5 × 108 | 1.2 × 109 | 8.85 (0.38) | 7.15 × 108 |
| After purification, in PBMCs for spiking | 6.38 × 106 | 5.62 × 107 | 6.33 × 107 | 5.86 × 108 | 8.78 × 107 | 1.51 × 108 | 7.87 (0.72) | 7.49 × 107 |
| % CMV recovery‡ | 3.54 | 11 | 4.22 | 32.6 | 19.5 | 12.6 | — | — |
| CMV signal in final spike attributable to fibroblasts | 2.4 × 106 | 1.44 × 106 | 6.6 × 106 | 2.9 × 106 | 9.6 × 106 | 2.4 × 105 | 5.86 (1.13) | 7.31 × 105 |
| % CMV from fibroblasts§ | 37.7 | 2.56 | 0.01 | 0.51 | 10.9 | 0.16 | — | — |
CMV copies . | A . | B . | C . | D . | E . | F . | Mean log copy no. (SD) . | Mean copy no. . |
|---|---|---|---|---|---|---|---|---|
| Spiking samples for whole blood units | ||||||||
| After coculture | 1.64 × 109 | 2.52 × 108 | 1.42 × 109 | 5.92 × 108 | 1.53 × 109 | 2.75 × 108 | 8.86 (0.38) | 7.26 × 108 |
| After purification, in PBMCs for spiking | 5.08 × 107 | 4.69 × 106 | 2.90 × 107 | 3.70 × 107 | 2.50 × 107 | 3.19 × 106 | 7.22 (0.50) | 1.65 × 107 |
| % CMV recovery* | 3.1 | 1.86 | 2.04 | 6.25 | 1.63 | 1.16 | — | — |
| CMV signal in final spike attributable to fibroblasts | 3.3 × 105 | 7.5 × 105 | 9.9 × 103 | 8.1 × 103 | 8.1 × 102 | 1.2 × 104 | 4.38 (1.11) | 2.4 × 104 |
| % CMV from fibroblasts† | 0.65 | 16 | 0.03 | 0.02 | 0.003 | 0.37 | — | — |
| Spiking samples for platelet units | ||||||||
| After coculture | 1.8 × 108 | 5.1 × 108 | 1.5 × 109 | 1.8 × 109 | 4.5 × 108 | 1.2 × 109 | 8.85 (0.38) | 7.15 × 108 |
| After purification, in PBMCs for spiking | 6.38 × 106 | 5.62 × 107 | 6.33 × 107 | 5.86 × 108 | 8.78 × 107 | 1.51 × 108 | 7.87 (0.72) | 7.49 × 107 |
| % CMV recovery‡ | 3.54 | 11 | 4.22 | 32.6 | 19.5 | 12.6 | — | — |
| CMV signal in final spike attributable to fibroblasts | 2.4 × 106 | 1.44 × 106 | 6.6 × 106 | 2.9 × 106 | 9.6 × 106 | 2.4 × 105 | 5.86 (1.13) | 7.31 × 105 |
| % CMV from fibroblasts§ | 37.7 | 2.56 | 0.01 | 0.51 | 10.9 | 0.16 | — | — |
The proportion of CMV signal attributable to fibroblast contamination is also shown. A mean of 3 CMV copies per fibroblast is used in the calculation.—indicates not applicable.
Mean % (SD): *2.67 (1.87); †0.65 (1.09); ‡13.9 (10.9); §8.64 (14.8).