Table 5. mtDNA heterogeneity... | American Society of Hematology

Table 5.

mtDNA heterogeneity characteristics of CD34⁺ clones from adult BM and CB

	Adult BM CD34⁺	CB CD34⁺
Plating efficiency, %	30.0	78.6*
Aggregate cell genotype	Uniform pattern	Frequent mixed nucleotide signal†
Heterogeneity of CD34⁺ clones, %
Total rate	24.9*	1.6
Unique pattern	7.5*	1.2
Substitution (no.)	10.5 (64 of 611)*	0.0 (0 of 580)
Poly C tract (no.)†	11.9 (73 of 611)*	1.2 (7 of 580)
Length heteroplasmy at 303 to 315	10.8 (66 of 611)*	0.0 (0 of 580)
Length heteroplasmy at 16184 to 16193	1.1 (7 of 611)	0.5 (3 of 580)
Nucleotide change	0.0 (0 of 611)	0.7 (4 of 580)
Substitution plus poly C tract and others (no.)	2.5 (15 of 611)	0.4 (2 of 580)

	Adult BM CD34⁺	CB CD34⁺
Plating efficiency, %	30.0	78.6*
Aggregate cell genotype	Uniform pattern	Frequent mixed nucleotide signal†
Heterogeneity of CD34⁺ clones, %
Total rate	24.9*	1.6
Unique pattern	7.5*	1.2
Substitution (no.)	10.5 (64 of 611)*	0.0 (0 of 580)
Poly C tract (no.)†	11.9 (73 of 611)*	1.2 (7 of 580)
Length heteroplasmy at 303 to 315	10.8 (66 of 611)*	0.0 (0 of 580)
Length heteroplasmy at 16184 to 16193	1.1 (7 of 611)	0.5 (3 of 580)
Nucleotide change	0.0 (0 of 611)	0.7 (4 of 580)
Substitution plus poly C tract and others (no.)	2.5 (15 of 611)	0.4 (2 of 580)

Data are expressed as mutations per total assayed CD34⁺ clones.

Statistically significant difference (P < .05) between 2 groups.

Homopolymeric C tracts at nucleotides position at 303 to 315 and 16184 to 16193 in HV2 and HV1, respectively.