Table 4.

Summary of mtDNA heterogeneity among individual single CD34+ clones from normal adult bone marrow and cord blood

Heterogeneity
Different
Unique
Donor and mtDNA sequence
Clone no.
No.
%
No.
%
BM donor 1      
    “Aggregate” sequence of total BM cells   85   0   0   0   0  
    Nonaggregate sequences    30   26.1   8   7.0  
        +8CT6C*, 9CT6C*  22      
        +9CT6C*  2      
        +7CT6C*  1      
        +189A>G/A   1      
        +204T>C   1      
        +277C>T   1      
        +514insCA   1      
        +16114C>T   1      
BM donor 2      
    “Aggregate” sequence of total BM cells   27   0   0   0   0  
    Nonaggregate sequences    9   25.0   5   13.9  
        +16184C>CC (11C)*  5      
        +16131T>C/T   1      
        +16145G>A   1      
        +16184C>CCCC (13C)*  1      
        73A>G, 263A>G, 191A>AA, 194C>T, 199T>C, 207G>A, 8CT6C*, 489T>C, 16147C>T, 16173C>T, 16245C>T, 16362T>C   1      
BM donor 3      
    “Aggregate” sequence of total BM cells   96   0   0   0   0  
    Nonaggregate sequences    24   20.0   7   5.8  
        +9CT6C*, 10CT6C*  11      
        +9CT6C*  6      
        +8CT6C*  2      
        +182C>T/C, 8CT6C*, 9CT6C*  2      
        +71delG, 9CT6C*, 10CT6C*  1      
        +279T>C/T   1      
        +16153G>A   1      
BM donor 4      
    “Aggregate” sequence of total BM cells   92   0   0   0   0  
    Nonaggregate sequences    19   17.1   6   5.4  
        +8CT6C*, 9CT6C*  11      
        +514-515delCA, 514insCA†  3      
        +7CT6C*, 8CT6C*  2      
        +9CT6C*, 10CT6C*  1      
        +89T>C   1      
        +8CT6C*, 9CT6C*, 16093T‡  1      
BM donor 5      
    “Aggregate” sequence of total BM cells   57   0   0   0   0  
    Nonaggregate sequences    57   50.0   14   12.3  
        +514insCA‡  19      
        +264C>T   18      
        +514-515delCA, 514insCA†  5      
        +264C>T/C   3      
        +7CT6C*, 8CT6C*  2      
        +146T>C, 514insCA‡  2      
        +146T>C, 264C>T/C   1      
        +146T>C/T, 514insCA‡  1      
        +146T>C   1      
        +189A>G   1      
        +264C>T/C, 514insCA‡  1      
        +161T>C/T, 264C>T, 514insCA‡  1      
        +16189T>C*  1      
        +16296C>C/T   1      
BM donor 6      
    “Aggregate” sequence of total BM cells   102   0   0   0   0  
    Nonaggregate sequences    13   11.3   6   5.2  
        +8CT6C*, 9CT6C*  5      
        +200A>G/A   3      
        +200A>G   2      
        +200A>G/A, 7CT6C*, 8CT6C*  1      
        +200A>G/A, 8CT6C*, 9CT6C*  1      
        +7CT6C*, 8CT6C*  1      
CB donor 1      
    “Aggregate” sequence of total CB cells   118   0   0   0   0  
    73A>G, 153A>G, 183A>G, 263A>G, 7CT6C*, 8CT6C*, 325C>T, 463C>T, 485T>C, 489T>C, 514-515delCA, 16198T>C, 16223C>T, 16268C>T, 16354C>T, 16381T>A, 16519T>C   1   1   0.8   1   0.8  
CB donor 2      
    “Aggregate” sequence of total CB cells   120   0   0   0   0  
CB donor 3      
    “Aggregate” sequence of total CB cells   100   0   0   0   0  
    Nonaggregate sequences    4   3.8   2   1.9  
        +316T>G/C   3      
        +305C>A, 306C>A, 307C>A   1      
CB donor 4      
    “Aggregate” sequence of total CB cells   118   0   0   0   0  
    +6CT6C*, 16022T>C   1   1   0.8   1   0.8  
CB donor 5      
    “Aggregate” sequence of total CB cells   115   0   0   0   0  
    Nonaggregate sequences    3   2.5   3   2.5  
        +16184C>CCC (12C)*  1      
        +16189T>C*  1      
        +16184C>CCCC (13C)*
 		 1
 
 
 
 
 
Heterogeneity
Different
Unique
Donor and mtDNA sequence
Clone no.
No.
%
No.
%
BM donor 1      
    “Aggregate” sequence of total BM cells   85   0   0   0   0  
    Nonaggregate sequences    30   26.1   8   7.0  
        +8CT6C*, 9CT6C*  22      
        +9CT6C*  2      
        +7CT6C*  1      
        +189A>G/A   1      
        +204T>C   1      
        +277C>T   1      
        +514insCA   1      
        +16114C>T   1      
BM donor 2      
    “Aggregate” sequence of total BM cells   27   0   0   0   0  
    Nonaggregate sequences    9   25.0   5   13.9  
        +16184C>CC (11C)*  5      
        +16131T>C/T   1      
        +16145G>A   1      
        +16184C>CCCC (13C)*  1      
        73A>G, 263A>G, 191A>AA, 194C>T, 199T>C, 207G>A, 8CT6C*, 489T>C, 16147C>T, 16173C>T, 16245C>T, 16362T>C   1      
BM donor 3      
    “Aggregate” sequence of total BM cells   96   0   0   0   0  
    Nonaggregate sequences    24   20.0   7   5.8  
        +9CT6C*, 10CT6C*  11      
        +9CT6C*  6      
        +8CT6C*  2      
        +182C>T/C, 8CT6C*, 9CT6C*  2      
        +71delG, 9CT6C*, 10CT6C*  1      
        +279T>C/T   1      
        +16153G>A   1      
BM donor 4      
    “Aggregate” sequence of total BM cells   92   0   0   0   0  
    Nonaggregate sequences    19   17.1   6   5.4  
        +8CT6C*, 9CT6C*  11      
        +514-515delCA, 514insCA†  3      
        +7CT6C*, 8CT6C*  2      
        +9CT6C*, 10CT6C*  1      
        +89T>C   1      
        +8CT6C*, 9CT6C*, 16093T‡  1      
BM donor 5      
    “Aggregate” sequence of total BM cells   57   0   0   0   0  
    Nonaggregate sequences    57   50.0   14   12.3  
        +514insCA‡  19      
        +264C>T   18      
        +514-515delCA, 514insCA†  5      
        +264C>T/C   3      
        +7CT6C*, 8CT6C*  2      
        +146T>C, 514insCA‡  2      
        +146T>C, 264C>T/C   1      
        +146T>C/T, 514insCA‡  1      
        +146T>C   1      
        +189A>G   1      
        +264C>T/C, 514insCA‡  1      
        +161T>C/T, 264C>T, 514insCA‡  1      
        +16189T>C*  1      
        +16296C>C/T   1      
BM donor 6      
    “Aggregate” sequence of total BM cells   102   0   0   0   0  
    Nonaggregate sequences    13   11.3   6   5.2  
        +8CT6C*, 9CT6C*  5      
        +200A>G/A   3      
        +200A>G   2      
        +200A>G/A, 7CT6C*, 8CT6C*  1      
        +200A>G/A, 8CT6C*, 9CT6C*  1      
        +7CT6C*, 8CT6C*  1      
CB donor 1      
    “Aggregate” sequence of total CB cells   118   0   0   0   0  
    73A>G, 153A>G, 183A>G, 263A>G, 7CT6C*, 8CT6C*, 325C>T, 463C>T, 485T>C, 489T>C, 514-515delCA, 16198T>C, 16223C>T, 16268C>T, 16354C>T, 16381T>A, 16519T>C   1   1   0.8   1   0.8  
CB donor 2      
    “Aggregate” sequence of total CB cells   120   0   0   0   0  
CB donor 3      
    “Aggregate” sequence of total CB cells   100   0   0   0   0  
    Nonaggregate sequences    4   3.8   2   1.9  
        +316T>G/C   3      
        +305C>A, 306C>A, 307C>A   1      
CB donor 4      
    “Aggregate” sequence of total CB cells   118   0   0   0   0  
    +6CT6C*, 16022T>C   1   1   0.8   1   0.8  
CB donor 5      
    “Aggregate” sequence of total CB cells   115   0   0   0   0  
    Nonaggregate sequences    3   2.5   3   2.5  
        +16184C>CCC (12C)*  1      
        +16189T>C*  1      
        +16184C>CCCC (13C)*
 		 1
 
 
 
 
 

Different indicates different from aggregate cell sequence; unique, uniquely different heterogeneity; +, mtDNA nucleotide changes in comparison with aggregate cell mtDNA sequence.

*

Homopolymeric C tracts at nucleotides position at 303 to 315 (for example, 8CT6C defined CCCCCCCCTCCCCCC) and 16184 to 16193 in HV2 and HV1, respectively.

†

Mixed pattern of 514-515delCA and 514insCA.

‡

The same as the Cambridge Reference Sequence but different from the aggregate sequence.

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