Table 1. FISH and immunostaining results... | American Society of Hematology

Table 1.

FISH and immunostaining results

			Lamina propria							Epithelium
	Days from BMT					Y CD45⁺				Y CD45⁺		Y CD45^-
Controls and patients	Days from BMT		GVHD grade	X signals	Y signals	Y CD45⁺ signals	Y %	X signals	Y signals	Y CD45⁺ signals	Y %	Y CD45^- signals	Y %
Control
Positive	18	1	81	73	44	60	75	82	4	5	78	95
Negative	861	1	155	0	0	—	161	0	0	—	0	—
Patient
1	30	1	100	5	3	60	141	0	0	—	0	—
2	22	2	128	18	15	83	154	0.02	0.02	100	0	0
3	14	2	137	11	8	72	144	0.02	0.02	100	0	0
4	30	2	142	6	4	66	152	0.04	0.04	100	0	0
5	30	2	151	3	2	66	165	0.08	0.04	50	0.04	50
6	33	3	177	36	25	69	191	0.16	0.16	100	0	0

			Lamina propria							Epithelium
	Days from BMT					Y CD45⁺				Y CD45⁺		Y CD45^-
Controls and patients	Days from BMT		GVHD grade	X signals	Y signals	Y CD45⁺ signals	Y %	X signals	Y signals	Y CD45⁺ signals	Y %	Y CD45^- signals	Y %
Control
Positive	18	1	81	73	44	60	75	82	4	5	78	95
Negative	861	1	155	0	0	—	161	0	0	—	0	—
Patient
1	30	1	100	5	3	60	141	0	0	—	0	—
2	22	2	128	18	15	83	154	0.02	0.02	100	0	0
3	14	2	137	11	8	72	144	0.02	0.02	100	0	0
4	30	2	142	6	4	66	152	0.04	0.04	100	0	0
5	30	2	151	3	2	66	165	0.08	0.04	50	0.04	50
6	33	3	177	36	25	69	191	0.16	0.16	100	0	0

FISH signals were counted as follows: X signals on 500 cells in epithelium and lamina propria; Y signals on 500 cells in lamina propria and on 5000 cells in epithelium. All results included in this table are related to 100 cells. BMT indicates bone marrow transplantation; —, not applicable.

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