CCL 16-induced HUVEC chemotaxis
. | Top chamber . | . | . | . | |||
---|---|---|---|---|---|---|---|
Bottom chamber . | No. of migrated cells . | . | . | . | |||
CCL 16-Cos, ng/mL . | 0 . | 5 . | 25 . | 50 . | |||
0 | 21 ± 4 | 22 ± 3 | 23 ± 3 | 24 ± 5 | |||
5 | 30 ± 5 | 35 ± 5 | 31 ± 3 | 30 ± 6 | |||
25 | 47 ± 5 | 43 ± 7 | 56 ± 4 | 50 ± 5 | |||
50 | 58 ± 3 | 55 ± 5 | 63 ± 4 | 68 ± 4 |
. | Top chamber . | . | . | . | |||
---|---|---|---|---|---|---|---|
Bottom chamber . | No. of migrated cells . | . | . | . | |||
CCL 16-Cos, ng/mL . | 0 . | 5 . | 25 . | 50 . | |||
0 | 21 ± 4 | 22 ± 3 | 23 ± 3 | 24 ± 5 | |||
5 | 30 ± 5 | 35 ± 5 | 31 ± 3 | 30 ± 6 | |||
25 | 47 ± 5 | 43 ± 7 | 56 ± 4 | 50 ± 5 | |||
50 | 58 ± 3 | 55 ± 5 | 63 ± 4 | 68 ± 4 |
Checkerboard analysis of CCL 16-induced HUVEC chemotaxis was evaluated by adding CCL 16-Cos at specified concentrations in the bottom compartment of the Boyden chamber in the absence or presence of specified concentrations of the chemokine added in the top compartment of the apparatus. Mean ± SD of 3 experiments.