Table 1.
Apoptotic effect of a chemical series of RRMs in NB4 cells
Compound | 1 μM | 10 μM | 100 μM | |
|---|---|---|---|---|
| ST1926 |  | 72.0 ± 2.3* | > 95 | > 95 |
| CD437 |  | 35.1 ± 3.0* | 80.0 ± 2.5* | > 95 |
| ST1898 |  | 5.0 ± 2.6 | 25.7 ± 6.5* | 40.1 ± 16.5* |
| ST1879 |  | 2.5 ± 0.8 | 33.3 ± 19.7* | > 95 |
| ST2474 |  | 9.0 ± 4.1 | 58.8 ± 1.8* | 88.2 ± 14.3* |
| ST2306 |  | 5.6 ± 0.1 | 43.9 ± 2.3* | 93.3 ± 1.9* |
| ST2307 |  | 6.2 ± 3.0 | 41.6 ± 4.6* | 46.5 ± 3.8* |
| ST2142 |  | < 1 | 9.9 ± 6.7* | 48.4 ± 6.6* |
| ST2064 |  | < 1 | 2.2 ± 1.7 | 70.4 ± 18.1* |
| ST2475 |  | < 1 | 1.6 ± 1.6 | 26.6 ± 5.9* |
| ST1927 |  | 3.0 ± 1.0 | 2.9 ± 0.8 | 4.1 ± 2.6* |
| ST2016 |  | < 1 | < 1 | Necrosis |
| ST2060 |  | < 1 | < 1 | 1.8 ± 1.3 |
| ST2062 |  | < 1 | < 1 | 1.7 ± 0.8 |
| ST2065 |  | < 1 | < 1 | 1.3 ± 0.3 |
| ST2067 |  | < 1 | 1.1 ± 0.4 | < 1 |
Compound | 1 μM | 10 μM | 100 μM | |
|---|---|---|---|---|
| ST1926 | | 72.0 ± 2.3* | > 95 | > 95 |
| CD437 | | 35.1 ± 3.0* | 80.0 ± 2.5* | > 95 |
| ST1898 | | 5.0 ± 2.6 | 25.7 ± 6.5* | 40.1 ± 16.5* |
| ST1879 | | 2.5 ± 0.8 | 33.3 ± 19.7* | > 95 |
| ST2474 | | 9.0 ± 4.1 | 58.8 ± 1.8* | 88.2 ± 14.3* |
| ST2306 | | 5.6 ± 0.1 | 43.9 ± 2.3* | 93.3 ± 1.9* |
| ST2307 | | 6.2 ± 3.0 | 41.6 ± 4.6* | 46.5 ± 3.8* |
| ST2142 | | < 1 | 9.9 ± 6.7* | 48.4 ± 6.6* |
| ST2064 | | < 1 | 2.2 ± 1.7 | 70.4 ± 18.1* |
| ST2475 | | < 1 | 1.6 ± 1.6 | 26.6 ± 5.9* |
| ST1927 | | 3.0 ± 1.0 | 2.9 ± 0.8 | 4.1 ± 2.6* |
| ST2016 | | < 1 | < 1 | Necrosis |
| ST2060 | | < 1 | < 1 | 1.8 ± 1.3 |
| ST2062 | | < 1 | < 1 | 1.7 ± 0.8 |
| ST2065 | | < 1 | < 1 | 1.3 ± 0.3 |
| ST2067 | | < 1 | 1.1 ± 0.4 | < 1 |
The apoptotic index of each compound was measured at 3 different concentrations, as indicated, following incubation of NB4 cells for 4 hours. The notations < 1 and > 95 indicate an apototic index below the limit of detection of the assay and evidence of nuclear fragmentation in practically the totality of the cell population, respectively.
*
Significantly higher than NB4 cells treated with vehicle (P < .01 according to the Student t test). The apoptotic index in vehicle-treated NB4 cells varies between 3% and 5% (mean, n = 3) according to the experiment, with a standard deviation of less than or equal to 10% of the mean value.