Anti-BAFF and anti-APRIL antibodies restore apoptotic death in B-CLL cells
. | Patients . | . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 8B . | . | 15C . | . | 18B . | 19 . | 20 . | 21 . | Mean . | |||||||
| Antibody, μg/mL | 0.2 | 2 | 0.2 | 2 | 2 | 2 | 2 | 2 | — | |||||||
| Goat | ||||||||||||||||
| IgG | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||||||
| Anti-BAFF | 1.57 | 1.25 | 4.19 | 0.98 | 1.2 | 1.31 | 1.47 | 1.08 | 1.22* | |||||||
| Anti-APRIL | 1.45 | 1.38 | 5.37 | 0.92 | 0.95 | 1.67 | 1.33 | 0.99 | 1.21 | |||||||
| Rabbit | ||||||||||||||||
| IgG | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||||||
| Anti-BAFF | 1.58 | 1.1 | 2.13 | 1.25 | 1.59 | 1.41 | 4.75 | 2.11 | 2.04* | |||||||
. | Patients . | . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 8B . | . | 15C . | . | 18B . | 19 . | 20 . | 21 . | Mean . | |||||||
| Antibody, μg/mL | 0.2 | 2 | 0.2 | 2 | 2 | 2 | 2 | 2 | — | |||||||
| Goat | ||||||||||||||||
| IgG | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||||||
| Anti-BAFF | 1.57 | 1.25 | 4.19 | 0.98 | 1.2 | 1.31 | 1.47 | 1.08 | 1.22* | |||||||
| Anti-APRIL | 1.45 | 1.38 | 5.37 | 0.92 | 0.95 | 1.67 | 1.33 | 0.99 | 1.21 | |||||||
| Rabbit | ||||||||||||||||
| IgG | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||||||
| Anti-BAFF | 1.58 | 1.1 | 2.13 | 1.25 | 1.59 | 1.41 | 4.75 | 2.11 | 2.04* | |||||||
Leukemic B cells from 6 B-CLL patients were cultured for 72 hours in the presence of 0.2 or 2 μg/mL goat anti-BAFF and anti-APRIL antibodies or rabbit anti-BAFF antibody or their respective isotype controls (goat IgG or rabbit IgG). Results are expressed as the cytoplasm nucleosome enrichment, in comparison with the respective goat or rabbit isotype controls taken as 1.
Probability (.01 ≤ P < .05, for antibody concentration of 2 μg/mL) is expressed in comparison with the respective isotype controls.