BAFF and APRIL protect B-CLL cells from spontaneous apoptosis
. | Patients . | . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 6B . | 8B . | 13C . | 15C . | 18B . | 19 . | 21 . | 22 . | 23 . | Mean . | ||||||||
| Control | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||||||
| BAFF | 0.45 | 0.69 | 0.43 | 0.5 | 0.85 | 0.2 | 0.79 | 0.6 | 0.43 | 0.55* | ||||||||
| APRIL | 0.43 | 0.85 | 0.59 | 0.69 | 0.55 | 0.09 | 0.98 | 0.78 | 0.16 | 0.57* | ||||||||
| BAFF + APRIL | ND | 0.51 | ND | ND | 0.74 | ND | 0.6 | ND | ND | 0.62† | ||||||||
. | Patients . | . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 6B . | 8B . | 13C . | 15C . | 18B . | 19 . | 21 . | 22 . | 23 . | Mean . | ||||||||
| Control | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||||||
| BAFF | 0.45 | 0.69 | 0.43 | 0.5 | 0.85 | 0.2 | 0.79 | 0.6 | 0.43 | 0.55* | ||||||||
| APRIL | 0.43 | 0.85 | 0.59 | 0.69 | 0.55 | 0.09 | 0.98 | 0.78 | 0.16 | 0.57* | ||||||||
| BAFF + APRIL | ND | 0.51 | ND | ND | 0.74 | ND | 0.6 | ND | ND | 0.62† | ||||||||
Cytoplasm nucleosome enrichment was measured after the addition of soluble BAFF and APRIL (0.25 μg/mL) to cultures of leukemic B cells for 72-hour incubation in the presence of medium alone for 9 patients with leukemia. Results are expressed as cytoplasm nucleosome enrichment compared with untreated cells taken as 1 (enrichment of nucleosomes in untreated cells increased approximately 5 times between days 0 and 3 of culture as a consequence of spontaneous apoptosis). Probability is expressed in comparison with the untreated cells (†). ND indicates not done.
.0001 ≤ P < .001.
.01 ≤ P < .05.