Table 1. Characteristics of PCR primers... | American Society of Hematology

Table 1.

Characteristics of PCR primers and allele-specific-oligonucleotides (ASO) used for genotyping MTHFR polymorphisms

Amino acid change	DNA variant	PCR primers, 5′ to 3′	Product size	Allele	ASO probe, 5′ to 3′*	Washing
Ala222Val	C677T	F: tgaaggagaaggtgtctgcgg	198 bp	C677	gcgggagCcgatttc	50°C
		R: aggacggtgcggtgagagtg		T677	gcgggagTcgatttc	50°C
Glu428Ala	A1298C	F: ctttggggagctgaaggacta	163 bp	A1298	ccagtgaagAaagtg	48°C
		R: cactttgtgaccattccggttt		C1298	ccagtgaagCaagtg	50°C

Amino acid change	DNA variant	PCR primers, 5′ to 3′	Product size	Allele	ASO probe, 5′ to 3′*	Washing
Ala222Val	C677T	F: tgaaggagaaggtgtctgcgg	198 bp	C677	gcgggagCcgatttc	50°C
		R: aggacggtgcggtgagagtg		T677	gcgggagTcgatttc	50°C
Glu428Ala	A1298C	F: ctttggggagctgaaggacta	163 bp	A1298	ccagtgaagAaagtg	48°C
		R: cactttgtgaccattccggttt		C1298	ccagtgaagCaagtg	50°C

Uppercase character indicates the polymorphic position.