Characterization of GeneChip small sample target labeling assay
Cell source . | Total RNA used initially . | cRNA yield, μg . | % of “present” call . | 3′/5′ ratio, GAPDH . |
|---|---|---|---|---|
| N CD4 | 500 ng | 54.2 | 44.4 | 2.13 |
| N CD4 | 500 ng | 53.4 | 47.7 | 2.84 |
| N CD4* | 5 μg | 52.2 | 46.7 | 1.83 |
| N CD4* | 5 μg | 54.3 | 44.9 | 1.44 |
| Pool-AA1-1 | 500 ng | 51.8 | 45.4 | 2.17 |
| Pool-AA1-2 | 500 ng | 49.5 | 46.6 | 2.68 |
| Pool-AA1-3 | 500 ng | 50.6 | 44.1 | 2.67 |
| Pool-AA2 | 500 ng | 50.6 | 48.5 | 3.27 |
| Pool-N1-1 | 500 ng | 53.3 | 41.9 | 2.55 |
| Pool-N1-2 | 500 ng | 51.7 | 44.7 | 1.92 |
| Pool-N1-3 | 500 ng | 54.8 | 47.0 | 1.94 |
| Pool-N2 | 500 ng | 48.9 | 46.7 | 2.80 |
Cell source . | Total RNA used initially . | cRNA yield, μg . | % of “present” call . | 3′/5′ ratio, GAPDH . |
|---|---|---|---|---|
| N CD4 | 500 ng | 54.2 | 44.4 | 2.13 |
| N CD4 | 500 ng | 53.4 | 47.7 | 2.84 |
| N CD4* | 5 μg | 52.2 | 46.7 | 1.83 |
| N CD4* | 5 μg | 54.3 | 44.9 | 1.44 |
| Pool-AA1-1 | 500 ng | 51.8 | 45.4 | 2.17 |
| Pool-AA1-2 | 500 ng | 49.5 | 46.6 | 2.68 |
| Pool-AA1-3 | 500 ng | 50.6 | 44.1 | 2.67 |
| Pool-AA2 | 500 ng | 50.6 | 48.5 | 3.27 |
| Pool-N1-1 | 500 ng | 53.3 | 41.9 | 2.55 |
| Pool-N1-2 | 500 ng | 51.7 | 44.7 | 1.92 |
| Pool-N1-3 | 500 ng | 54.8 | 47.0 | 1.94 |
| Pool-N2 | 500 ng | 48.9 | 46.7 | 2.80 |
GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase; N, healthy donor.
Standard protocol.