Examples of genes up-regulated in EphB4-/- EBs by DNA array analysis
Gene name . | Annotation . |
|---|---|
| Developmental genes | |
| cerebellum2 | Zinc-finger transcription factor |
| cerebellum4 | Zinc-finger transcription factor |
| α-internexin | Neuronal intermediate filament protein |
| nestin | Neuroectodermal marker |
| N-CAM1 | Neural cell adhesion molecular, neuroectodermal marker |
| N-CAM2 | Neural cell adhesion molecular |
| plp | Myelin proteolipid protein |
| Nfl | Neurofilament-medium |
| GAD | Glutamic acid decarboxylase |
Gene name . | Annotation . |
|---|---|
| Developmental genes | |
| cerebellum2 | Zinc-finger transcription factor |
| cerebellum4 | Zinc-finger transcription factor |
| α-internexin | Neuronal intermediate filament protein |
| nestin | Neuroectodermal marker |
| N-CAM1 | Neural cell adhesion molecular, neuroectodermal marker |
| N-CAM2 | Neural cell adhesion molecular |
| plp | Myelin proteolipid protein |
| Nfl | Neurofilament-medium |
| GAD | Glutamic acid decarboxylase |
Day-4 EBs of EphB4+/- and EphB4-/- were used for DNA microarray analysis. Affymetrix mouse U74Av2 chips (about 12 000 genes) were hybridized on a GeneChip system (Affymetrix) at the Center for Genomics Research of Harvard University. Scanned chip images were analyzed using the Microarray Suite Software version 5.0 (Affymetrix). A transcript was considered “change” when the fold change was at least 2. RNA samples for microarray were generated from 4 independent experiments.