Oligonucleotide primers used for RT-PCR
Gene . | Primer sequence . | Tm °C . | Size, bp . |
|---|---|---|---|
| EphB4 | Sense 5′-TGGCTGATCACGAACTTGACCTAC-3′ | 61 | 323 |
| Antisense 5′-AGGCAGGACTCGTCTCCTATTT-3′ | 58 | ||
| EphB2 | Sense 5′-TAGTCTTCCTCATCGCTGTG-3′ | 53 | 366 |
| Antisense 5′-ATGATGGATGCCTCACTCAG-3′ | 54 | ||
| EphB3 | Sense 5′-AAGACTATCGGTTACCACCC-3′ | 53 | 354 |
| Antisense 5′-GTGACCCCAATCCTTAGCAG-3′ | 56 | ||
| ephrinB2 | Sense 5′-TCAAGAATTCAGCCCTAACCTC-3′ | 57 | 440 |
| Antisense 5′-TGACGATGAAGATGATGCATCC-3′ | 59 | ||
| CXCR4 | Sense 5′-GGCTGTAGAGCGAGTGTTGC-3′ | 57 | 390 |
| Antisense 5′-GTAGAGGTTGACAGTGTAGAT-3′ | 44 | ||
| Flk-1 | Sense 5′-CACCTGGCACTCTCCACCTTC-3′ | 60 | 239 |
| Antisense 5′-GATTTCATCCCACTACCGAA-3′ | 54 | ||
| CD31 | Sense 5′-GTCATGGCCATGGTCGAGTA-3′ | 58 | 261 |
| Antisense 5′-CTCCTCGGCGATCTTGCTGAA-3′ | 64 | ||
| β-H1 | Sense 5′-CTCAAGGAGACCTTTGCTCA-3′ | 54 | 309 |
| Antisense 5′-AGTCCCCATGGAGTCAAAGA-3′ | 56 | ||
| β-major | Sense 5′-TCCGATGAAGTTGGTGGTGA-3′ | 58 | 307 |
| Antisense 5′-AAATCCTTGCCAAGGTGGTG-3′ | 58 | ||
| Scl | Sense 5′-TATGAGATGGAGATTTCTGATG-3′ | 50 | 396 |
| Antisense 5′-GCTCCTCTGTGTAACTGTCC-3′ | 51 | ||
| GATA-2 | Sense 5′-GACGACAACCACCACCTTATG-3′ | 56 | 283 |
| Antisense 5′-GACTTAAAGGTGGGAGGTGTC-3′ | 54 | ||
| Oct-4 | Sense 5′-AAAGGTGTTCAGCCAGACCA-3′ | 56 | 369 |
| Antisense 5′-CTCATACTCTTCTCGTTGGG-3′ | 52 | ||
| Rex-1 | Sense 5′-CCAGTCCAGAATACCAGAGT-3′ | 50 | 338 |
| Antisense 5′-TGCAAGTAATGAGCTCGCCC-3′ | 60 | ||
| β-actin | Sense 5′-GATGACGATATCGCTGCGCTG-3′ | 63 | 439 |
| Antisense 5′-GTACGACCAGAGGCATACAGG-3′ | 56 |
Gene . | Primer sequence . | Tm °C . | Size, bp . |
|---|---|---|---|
| EphB4 | Sense 5′-TGGCTGATCACGAACTTGACCTAC-3′ | 61 | 323 |
| Antisense 5′-AGGCAGGACTCGTCTCCTATTT-3′ | 58 | ||
| EphB2 | Sense 5′-TAGTCTTCCTCATCGCTGTG-3′ | 53 | 366 |
| Antisense 5′-ATGATGGATGCCTCACTCAG-3′ | 54 | ||
| EphB3 | Sense 5′-AAGACTATCGGTTACCACCC-3′ | 53 | 354 |
| Antisense 5′-GTGACCCCAATCCTTAGCAG-3′ | 56 | ||
| ephrinB2 | Sense 5′-TCAAGAATTCAGCCCTAACCTC-3′ | 57 | 440 |
| Antisense 5′-TGACGATGAAGATGATGCATCC-3′ | 59 | ||
| CXCR4 | Sense 5′-GGCTGTAGAGCGAGTGTTGC-3′ | 57 | 390 |
| Antisense 5′-GTAGAGGTTGACAGTGTAGAT-3′ | 44 | ||
| Flk-1 | Sense 5′-CACCTGGCACTCTCCACCTTC-3′ | 60 | 239 |
| Antisense 5′-GATTTCATCCCACTACCGAA-3′ | 54 | ||
| CD31 | Sense 5′-GTCATGGCCATGGTCGAGTA-3′ | 58 | 261 |
| Antisense 5′-CTCCTCGGCGATCTTGCTGAA-3′ | 64 | ||
| β-H1 | Sense 5′-CTCAAGGAGACCTTTGCTCA-3′ | 54 | 309 |
| Antisense 5′-AGTCCCCATGGAGTCAAAGA-3′ | 56 | ||
| β-major | Sense 5′-TCCGATGAAGTTGGTGGTGA-3′ | 58 | 307 |
| Antisense 5′-AAATCCTTGCCAAGGTGGTG-3′ | 58 | ||
| Scl | Sense 5′-TATGAGATGGAGATTTCTGATG-3′ | 50 | 396 |
| Antisense 5′-GCTCCTCTGTGTAACTGTCC-3′ | 51 | ||
| GATA-2 | Sense 5′-GACGACAACCACCACCTTATG-3′ | 56 | 283 |
| Antisense 5′-GACTTAAAGGTGGGAGGTGTC-3′ | 54 | ||
| Oct-4 | Sense 5′-AAAGGTGTTCAGCCAGACCA-3′ | 56 | 369 |
| Antisense 5′-CTCATACTCTTCTCGTTGGG-3′ | 52 | ||
| Rex-1 | Sense 5′-CCAGTCCAGAATACCAGAGT-3′ | 50 | 338 |
| Antisense 5′-TGCAAGTAATGAGCTCGCCC-3′ | 60 | ||
| β-actin | Sense 5′-GATGACGATATCGCTGCGCTG-3′ | 63 | 439 |
| Antisense 5′-GTACGACCAGAGGCATACAGG-3′ | 56 |
Tm °C indicates annealing temperature.