Checkerboard analysis of EDN-induced DC migration
EDN in the lower wells (ng/mL) . | EDN in the upper wells, ng/mL . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | 0 . | 10 . | 100 . | 1000 . | |||
| 0 | 22 ± 3 | 24 ± 5 | 24 ± 5 | 22 ± 4 | |||
| 10 | 31 ± 5* | 22 ± 3 | 22 ± 5 | 22 ± 5 | |||
| 100 | 51 ± 5† | 35 ± 6* | 23 ± 4 | 20 ± 4 | |||
| 1000 | 105 ± 11† | 51 ± 7† | 36 ± 6* | 28 ± 4 | |||
EDN in the lower wells (ng/mL) . | EDN in the upper wells, ng/mL . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | 0 . | 10 . | 100 . | 1000 . | |||
| 0 | 22 ± 3 | 24 ± 5 | 24 ± 5 | 22 ± 4 | |||
| 10 | 31 ± 5* | 22 ± 3 | 22 ± 5 | 22 ± 5 | |||
| 100 | 51 ± 5† | 35 ± 6* | 23 ± 4 | 20 ± 4 | |||
| 1000 | 105 ± 11† | 51 ± 7† | 36 ± 6* | 28 ± 4 | |||
CD34+ cell-derived iDCs were used at 1 × 106/mL. EDN at specified concentrations was added to the lower wells of the chemotaxis chamber and DCs in the absence or presence of specified concentrations of EDN were added to the top wells of the chemotaxis chamber. The results are shown as the average (mean ± SD) of migrated DCs of triplicate wells (No./HPF).
P < .05 when compared with background DC migration (22 ± 3) by Mann-Whitney test
P < .001 when compared with background DC migration (22 ± 3) by Mann-Whitney test