RT-PCR of Eph mRNA
mRNA . | Sense primer . | Antisense primer . | Size, bp . |
|---|---|---|---|
| EphA2 | 241-259 CTCACACACCCGTATGGCAA | 976-957 GGCTCTCAGATGCCTCAAAC | 736 |
| EphA4 | 54-73 CGCCCTATTTTCGTGTCTCT | 666-647 CAGATTGCGGACTGTGAGTG | 613 |
| EphA7 | 1027-1046 GGGTTCTACAAGTCTTCCTC | 1538-1519 TCCTTCATTACTCCGCTCAC | 512 |
| EphB1 | 577-597 GTCATTGCCACCAAGAAGTC | 1015-996 CCACGCTGTTCTCAGGCTCA | 439 |
| EphB2 | 471-491 CACCAAGACCTTCCCCAACT | 905-887 GACGGTGCCATTCTCAACGG | 436 |
| EphB3 | 670-690 ACCACCGCA GGCTTCGCACT | 1408-1389 GGCGTAGTGTGGGCACTTCA | 739 |
| EphB6 | 824-843 TCCTGGTGTCTTCAGTTCTG | 1475-1456 GAAGCAAAGGATCGGAGCAC | 652 |
mRNA . | Sense primer . | Antisense primer . | Size, bp . |
|---|---|---|---|
| EphA2 | 241-259 CTCACACACCCGTATGGCAA | 976-957 GGCTCTCAGATGCCTCAAAC | 736 |
| EphA4 | 54-73 CGCCCTATTTTCGTGTCTCT | 666-647 CAGATTGCGGACTGTGAGTG | 613 |
| EphA7 | 1027-1046 GGGTTCTACAAGTCTTCCTC | 1538-1519 TCCTTCATTACTCCGCTCAC | 512 |
| EphB1 | 577-597 GTCATTGCCACCAAGAAGTC | 1015-996 CCACGCTGTTCTCAGGCTCA | 439 |
| EphB2 | 471-491 CACCAAGACCTTCCCCAACT | 905-887 GACGGTGCCATTCTCAACGG | 436 |
| EphB3 | 670-690 ACCACCGCA GGCTTCGCACT | 1408-1389 GGCGTAGTGTGGGCACTTCA | 739 |
| EphB6 | 824-843 TCCTGGTGTCTTCAGTTCTG | 1475-1456 GAAGCAAAGGATCGGAGCAC | 652 |
All Eph primers except EphB1 and EphB2 allowed distinctions in cDNA and genomic DNA.
bp = base pair.