Sequence analysis showing IgH ongoing rearrangement mechanisms in 2 cases of ALL
Sample . | VH-germ-line gene . | 3′-VH . | CDR3 (N-DH-N) . | 5′-JH germ-line gene . |
|---|---|---|---|---|
| Case 11 | ||||
| Diagnosis | VH 1-3 | TGTGCGAGA | AAGGGGGCATATTGTGGTGGTGACTGCTC (D2-21) | TACTAC (JH6) |
| Relapse | VH 2-21 | TGTGCACAC | CTTCGGG--------------------------- (D2-21) | ------ (JH6) |
| Case 449 | ||||
| Diagnosis | VH 4-34 | TGTGCTAGA | AAGGGATATTTTGACTGGTTATTATTTCTCGGAT (D3-9) | TACTAC (JH6) |
| Relapse | VH 3-33 | TGTGCGAGA | GATCGCG----------------------- (D3-9) | ------ (JH6) |
Sample . | VH-germ-line gene . | 3′-VH . | CDR3 (N-DH-N) . | 5′-JH germ-line gene . |
|---|---|---|---|---|
| Case 11 | ||||
| Diagnosis | VH 1-3 | TGTGCGAGA | AAGGGGGCATATTGTGGTGGTGACTGCTC (D2-21) | TACTAC (JH6) |
| Relapse | VH 2-21 | TGTGCACAC | CTTCGGG--------------------------- (D2-21) | ------ (JH6) |
| Case 449 | ||||
| Diagnosis | VH 4-34 | TGTGCTAGA | AAGGGATATTTTGACTGGTTATTATTTCTCGGAT (D3-9) | TACTAC (JH6) |
| Relapse | VH 3-33 | TGTGCGAGA | GATCGCG----------------------- (D3-9) | ------ (JH6) |
Germ-line identity is more than 99%. Germ-like D genes are boldfaced.