Angiogenic response to the MM endothelial cell or HUVEC conditioned medium (CM) assessed by the chick embryo chorioallantoic membrane (CAM)-gelatin sponge assay
. | . | . | No. of vessels at × 250 within 0.125 mm2 . | . | |
|---|---|---|---|---|---|
| Sponge loaded with . | No. of CM* . | No. of vessels at the sponge-CAM boundary at × 50 . | Inside the sponge . | At the sponge-CAM boundary . | |
| Negative control medium† | 10 | 11 ± 4‡ | 0 | 4 ± 2 | |
| Positive control medium | 10 | 38 ± 9 | 18 ± 7 | 25 ± 8 | |
| MM endothelial cell CM | 32 | 53 ± 15§ | 27 ± 10§ | 36 ± 12§ | |
| HUVEC CM | 20 | 21 ± 6§ | 1 ± 1§ | 6 ± 2§ | |
. | . | . | No. of vessels at × 250 within 0.125 mm2 . | . | |
|---|---|---|---|---|---|
| Sponge loaded with . | No. of CM* . | No. of vessels at the sponge-CAM boundary at × 50 . | Inside the sponge . | At the sponge-CAM boundary . | |
| Negative control medium† | 10 | 11 ± 4‡ | 0 | 4 ± 2 | |
| Positive control medium | 10 | 38 ± 9 | 18 ± 7 | 25 ± 8 | |
| MM endothelial cell CM | 32 | 53 ± 15§ | 27 ± 10§ | 36 ± 12§ | |
| HUVEC CM | 20 | 21 ± 6§ | 1 ± 1§ | 6 ± 2§ | |
Each tested on 2 to 3 eggs with similar results and expressed as mean.
The negative and positive control media were RPMI-1640 medium alone or containing bFGF (200 μg/mL), respectively.
Results are expressed as the final mean ± 1 SD.
P < .01 or better (analysis of variance by Fisher and Kruskal-Wallis test followed by Duncan [t], Bonferroni [t], and Wilcoxon paired tests).