Expression of vascular-associated cellular markers by endothelial cells from multiple myeloma (MM) bone marrow and human umbilical vein endothelial cells (HUVECs)
Marker . | Technique . | MM endothelial cells . | HUVECs . | P* . |
|---|---|---|---|---|
| Tie-2/Tek, OD† | Western blot | 2.71 ± 1.22† (52) | 0.04 ± 0.01 (18) | .0001 |
| VEGFR-1, OD | Western blot | 0.32 ± 0.12 (55) | 0.25 ± 0.16 (17) | NS |
| VEGFR-2, OD | Western blot | 1.78 ± 0.57 (48) | 0.37 ± 0.15 (22) | .001 |
| VEGFR-3, OD | Western blot | 0.18 ± 0.07 (57) | 0.08 ± 0.06 (22) | NS |
| bFGFR-1, OD | Western blot | 0.15 ± 0.03 (35) | 0.11 ± 0.02 (12) | NS |
| bFGFR-2, OD | Western blot | 1.12 ± 0.38 (49) | 0.08 ± 0.04 (18) | .0001 |
| bFGFR-3, OD | Western blot | 0.11 ± 0.08 (31) | 0.06 ± 0.05 (11) | NS |
| CD105, Endoglin, OD | Western blot | 1.56 ± 1.13 (57) | 0.47 ± 0.21 (19) | .01 |
| CD34, %‡ | FACS | 87 ± 25‡ (50) | 90 ± 18 (18) | NS |
| CD31, % | FACS | 96 ± 12 (52) | 97 ± 11 (20) | NS |
| CD133, AC133, % | FACS | 21 ± 15 (49) | 0.5 ± 0.2 (15) | .001 |
| Aquaporin 1, OD | Western blot | 2.15 ± 1.31 (57) | 0.97 ± 0.53 (22) | .01 |
| CD62E, E selectin, % | FACS | 84 ± 21 (55) | 65 ± 15 (20) | NS |
| CD61, β3 integrin, % | FACS | 78 ± 24 (42) | 55 ± 19 (12) | .05 |
| CD144, VE-cadherin, % | FACS | 81 ± 11 (45) | 72 ± 15 (12) | NS |
| CD138, syndecan-1, % | FACS | 82 ± 18 (37) | 88 ± 21 (12) | NS |
| Thrombospondin, OD | Western blot | 0.55 ± 0.33 (50) | 1.93 ± .68 (22) | .01 |
| EpoR, OD | Western blot | 2.63 ± 1.42 (57) | 2.31 ± 1.28 (18) | NS |
Marker . | Technique . | MM endothelial cells . | HUVECs . | P* . |
|---|---|---|---|---|
| Tie-2/Tek, OD† | Western blot | 2.71 ± 1.22† (52) | 0.04 ± 0.01 (18) | .0001 |
| VEGFR-1, OD | Western blot | 0.32 ± 0.12 (55) | 0.25 ± 0.16 (17) | NS |
| VEGFR-2, OD | Western blot | 1.78 ± 0.57 (48) | 0.37 ± 0.15 (22) | .001 |
| VEGFR-3, OD | Western blot | 0.18 ± 0.07 (57) | 0.08 ± 0.06 (22) | NS |
| bFGFR-1, OD | Western blot | 0.15 ± 0.03 (35) | 0.11 ± 0.02 (12) | NS |
| bFGFR-2, OD | Western blot | 1.12 ± 0.38 (49) | 0.08 ± 0.04 (18) | .0001 |
| bFGFR-3, OD | Western blot | 0.11 ± 0.08 (31) | 0.06 ± 0.05 (11) | NS |
| CD105, Endoglin, OD | Western blot | 1.56 ± 1.13 (57) | 0.47 ± 0.21 (19) | .01 |
| CD34, %‡ | FACS | 87 ± 25‡ (50) | 90 ± 18 (18) | NS |
| CD31, % | FACS | 96 ± 12 (52) | 97 ± 11 (20) | NS |
| CD133, AC133, % | FACS | 21 ± 15 (49) | 0.5 ± 0.2 (15) | .001 |
| Aquaporin 1, OD | Western blot | 2.15 ± 1.31 (57) | 0.97 ± 0.53 (22) | .01 |
| CD62E, E selectin, % | FACS | 84 ± 21 (55) | 65 ± 15 (20) | NS |
| CD61, β3 integrin, % | FACS | 78 ± 24 (42) | 55 ± 19 (12) | .05 |
| CD144, VE-cadherin, % | FACS | 81 ± 11 (45) | 72 ± 15 (12) | NS |
| CD138, syndecan-1, % | FACS | 82 ± 18 (37) | 88 ± 21 (12) | NS |
| Thrombospondin, OD | Western blot | 0.55 ± 0.33 (50) | 1.93 ± .68 (22) | .01 |
| EpoR, OD | Western blot | 2.63 ± 1.42 (57) | 2.31 ± 1.28 (18) | NS |
Results are expressed as mean ± 1 SD. Number of samples is shown in parentheses. NS indicates not significant.
Significance was assessed by analysis of variance by Fisher and Kruskal-Wallis test followed by Duncan [t], Bonferroni [t], and Wilcoxon paired tests.
Arbitrary optical density (OD) units (× 103) determined by Western blot and reading bands with Fluorad (described in “Patients, materials, and methods”).
Percentages of fluorescent cells over control antibody.