Viral peptide-specific CTLp CD8+ T-cell frequencies and frequencies of HCMV pp65 peptide-MHC class I-specific tetramer+ CD8+ CD3+ T cells in healthy donors and in alloSC transplant recipients
. | . | CTLp frequency per 106 CD8+ T cells* . | . | . | . | . | . | . | |
|---|---|---|---|---|---|---|---|---|---|
| Study no. . | Viral peptide . | Peptide labeled targets (mo after alloSCT) . | Negative control . | % HCMV pp65 tetramer+ CD8+ CD3+ T cells (mo after alloSCT) . | Absolute no. CD8+ CD3+ T cells × 109/L (mo after alloSCT) . | Absolute no. peptide-specific CTLp CD8+ T cells × 104/L . | Absolute no. HCMV pp65 tetramer + CD8+ CD3+ T cells × 104/L† . | Ratio of CTLp frequency to tetramer+ CD8+ CD3+ T -cells . | |
| 01 | |||||||||
| Donor | HCMV pp65495-503 | 1280 | 0 | 0.59 | 0.3 | 40 | 180 | 1:4.5 | |
| Recipient | HCMV pp65495-503 | 1040 (13) | 0 | 0.8 (13) | 80 | — | — | ||
| Recipient | HCMV pp65495-503 | 590 (24) | 0 | 0.74 (22) | 0.8 (24) | 50 | 600 | 1:12 | |
| Donor | HCMV IE72198-207 | 40 | 0 | — | 0.3 | 1 | — | — | |
| Recipient | HCMV IE72198-207 | 60 | 0 | — | 0.8 (13) | 5 | — | — | |
| Recipient | HCMV IE72198-207 | 20 | 0 | — | 0.8 (24) | 2 | — | — | |
| 02 | |||||||||
| Donor | HCMV pp65417-426 | 340 | 6 | 0.07 | 0.9 | 30 | 60 | 1:2 | |
| Recipient | HCMV pp65417-426 | 520 (5) | 6 | 0.05 (21) | 1.7 (5), 0.8 (15) | 90 | 40 | 1:0.4 | |
| Recipient | EBV EBNA3C880-891 | 220 | 6 | — | 1.7 (5) | 40 | — | — | |
| 03 | |||||||||
| Donor | HCMV pp65495-503 | 1280 | 3 | 0.4 | 0.5 | 60 | 200 | 1:3.3 | |
| Recipient | HCMV pp65495-503 | 2950 (36) | 180 | 0.9 (36) | 0.6 (36) | 180 | 540 | 1:3 | |
| 04 | |||||||||
| Donor | HCMV pp65495-503 | 950 | 12 | 0.24 | 0.8 | 80 | 190 | 1:2.4 | |
| Recipient | HCMV pp65495-503 | 170 (72) | 0 | 0.1 (72) | 1.6 (72) | 30 | 160 | 1:5.3 | |
| 05 | |||||||||
| Donor | HCMV pp65495-503 | ND | ND | Not detected | — | — | — | ||
| Recipient | HCMV pp65495-503 | 410 (before) | 0 | ND | 0.3 (before) | 10 | — | — | |
| Recipient | HCMV pp65495-503 | 1420 (21) | 23 | 0.56 (30) | 0.7 (21) | 100 | 390 | 1:3.9 | |
| 06 | |||||||||
| Donor | HCMV pp65495-503 | 4250 | 12 | 1.3 | 0.5 | 210 | 650 | 1:3.1 | |
| Recipient | HCMV pp65495-503 | Not detected (72) | 0 | Not detected (72) | 0.5 (72) | — | — | — | |
| Recipient | EBV EBNA3C281-290 | 1240 | 0 | — | 0.5 (72) | 60 | — | — | |
| 07 | |||||||||
| Donor | HCMV pp65417-426 | 2900 | 0 | 0.4 | 0.5 | 150 | 200 | 1:1.3 | |
| Donor | EBV EBNA3C880-891 | Bulk culture | Bulk culture | — | 0.5 | — | — | — | |
| 08 | |||||||||
| Donor | HCMV pp65495-503 | 2560 | 6 | 1.0 | 0.7 | 180 | 700 | 1:3.9 | |
| 09 | |||||||||
| Donor | EBV EBNA3C880-891 | ND | ND | — | 0.8 | — | — | — | |
| Recipient | EBV EBNA3C880-891 | 1040 (13) | 0 | — | 1.6 (13) | 170 | — | — | |
| 10 | |||||||||
| Donor | HCMV pp65495-503 | 560 | 0 | 0.4 | 0.9 | 50 | 360 | 1:7.2 | |
| Donor | HCMV pp65397-411 | 3300 | 0 | — | 0.9 | 300 | — | — | |
. | . | CTLp frequency per 106 CD8+ T cells* . | . | . | . | . | . | . | |
|---|---|---|---|---|---|---|---|---|---|
| Study no. . | Viral peptide . | Peptide labeled targets (mo after alloSCT) . | Negative control . | % HCMV pp65 tetramer+ CD8+ CD3+ T cells (mo after alloSCT) . | Absolute no. CD8+ CD3+ T cells × 109/L (mo after alloSCT) . | Absolute no. peptide-specific CTLp CD8+ T cells × 104/L . | Absolute no. HCMV pp65 tetramer + CD8+ CD3+ T cells × 104/L† . | Ratio of CTLp frequency to tetramer+ CD8+ CD3+ T -cells . | |
| 01 | |||||||||
| Donor | HCMV pp65495-503 | 1280 | 0 | 0.59 | 0.3 | 40 | 180 | 1:4.5 | |
| Recipient | HCMV pp65495-503 | 1040 (13) | 0 | 0.8 (13) | 80 | — | — | ||
| Recipient | HCMV pp65495-503 | 590 (24) | 0 | 0.74 (22) | 0.8 (24) | 50 | 600 | 1:12 | |
| Donor | HCMV IE72198-207 | 40 | 0 | — | 0.3 | 1 | — | — | |
| Recipient | HCMV IE72198-207 | 60 | 0 | — | 0.8 (13) | 5 | — | — | |
| Recipient | HCMV IE72198-207 | 20 | 0 | — | 0.8 (24) | 2 | — | — | |
| 02 | |||||||||
| Donor | HCMV pp65417-426 | 340 | 6 | 0.07 | 0.9 | 30 | 60 | 1:2 | |
| Recipient | HCMV pp65417-426 | 520 (5) | 6 | 0.05 (21) | 1.7 (5), 0.8 (15) | 90 | 40 | 1:0.4 | |
| Recipient | EBV EBNA3C880-891 | 220 | 6 | — | 1.7 (5) | 40 | — | — | |
| 03 | |||||||||
| Donor | HCMV pp65495-503 | 1280 | 3 | 0.4 | 0.5 | 60 | 200 | 1:3.3 | |
| Recipient | HCMV pp65495-503 | 2950 (36) | 180 | 0.9 (36) | 0.6 (36) | 180 | 540 | 1:3 | |
| 04 | |||||||||
| Donor | HCMV pp65495-503 | 950 | 12 | 0.24 | 0.8 | 80 | 190 | 1:2.4 | |
| Recipient | HCMV pp65495-503 | 170 (72) | 0 | 0.1 (72) | 1.6 (72) | 30 | 160 | 1:5.3 | |
| 05 | |||||||||
| Donor | HCMV pp65495-503 | ND | ND | Not detected | — | — | — | ||
| Recipient | HCMV pp65495-503 | 410 (before) | 0 | ND | 0.3 (before) | 10 | — | — | |
| Recipient | HCMV pp65495-503 | 1420 (21) | 23 | 0.56 (30) | 0.7 (21) | 100 | 390 | 1:3.9 | |
| 06 | |||||||||
| Donor | HCMV pp65495-503 | 4250 | 12 | 1.3 | 0.5 | 210 | 650 | 1:3.1 | |
| Recipient | HCMV pp65495-503 | Not detected (72) | 0 | Not detected (72) | 0.5 (72) | — | — | — | |
| Recipient | EBV EBNA3C281-290 | 1240 | 0 | — | 0.5 (72) | 60 | — | — | |
| 07 | |||||||||
| Donor | HCMV pp65417-426 | 2900 | 0 | 0.4 | 0.5 | 150 | 200 | 1:1.3 | |
| Donor | EBV EBNA3C880-891 | Bulk culture | Bulk culture | — | 0.5 | — | — | — | |
| 08 | |||||||||
| Donor | HCMV pp65495-503 | 2560 | 6 | 1.0 | 0.7 | 180 | 700 | 1:3.9 | |
| 09 | |||||||||
| Donor | EBV EBNA3C880-891 | ND | ND | — | 0.8 | — | — | — | |
| Recipient | EBV EBNA3C880-891 | 1040 (13) | 0 | — | 1.6 (13) | 170 | — | — | |
| 10 | |||||||||
| Donor | HCMV pp65495-503 | 560 | 0 | 0.4 | 0.9 | 50 | 360 | 1:7.2 | |
| Donor | HCMV pp65397-411 | 3300 | 0 | — | 0.9 | 300 | — | — | |