Summary of virus-specific CD8+ T-cell clones obtained in healthy donors and in alloSCT recipients
Study no. . | Viral peptide . | HLA restriction . | Number of clones with the same TCR Vβ sequence . | T-cell receptor . | β chain hypervariable nucleotide sequences of virus-specific CD8+ T-cell clones . |
|---|---|---|---|---|---|
| 01 | |||||
| Donor | HCMV pp65495-503 | A0201 | 2/2 | Clone 1.1 | Vβ14.1...gag ggg t... Jβ1.1 Cβ1 |
| Recipient | HCMV pp65495-503 | A0201 | 3/3 | Clone 1.3 | Vβ14.1...gag ggg t... Jβ1.1 Cβ1 |
| Donor | HCMV IE72198-207 | B0801 | 1/1 | Clone 1.2 | Vβ13.1...ggc ccc cta ccc ggg g... Jβ2.1 Cβ1 |
| Recipient | HCMV IE72198-207 | B0801 | 2/2 | Clone 1.4 | Vβ14.1...cc cag aga cgg gat t... Jβ1.1 Cβ1 |
| 02 | |||||
| Donor | HCMV pp65417-426 | B0702 | 3/3 | Clone 2.1 | Vβ6.4....t cgg ggg gag ggc cga ggg g... Jβ2.1 Cβ2 |
| Recipient | HCMV pp65417-426 | B0702 | 2/2 | Clone 2.2 | Vβ6.4....t cgg ggg gag ggc cga ggg g... Jβ2.1 Cβ2 |
| Recipient | EBV EBNA3C880-891 | B0702 | 2/2 | Clone 2.3 | Vβ14.1...c att cag gga g... Jβ1.2 Cβ1 |
| 03 | |||||
| Donor | HCMV pp65495-503 | A0201 | \(\left\{\begin{array}{l}3/4\\1/4\end{array}\right\}\) | Clone 3.1a | Vβ14.1...c cca ggc gcc ccg ggt... Jβ1.6 Cβ1 |
| Clone 3.1b | Vβ14.1...acg gcc ggg agc cct ta... Jβ2.7 Cβ2 | ||||
| Recipient | HCMV pp65495-503 | A0201 | \(\left\{\begin{array}{l}6/8\\2/8\end{array}\right\}\) | Clone 3.2a | Vβ14.1...c cca ggc gcc ccg ggt... Jβ1.6 Cβ1 |
| Clone 3.2b | Vβ14.1...acg gcc ggg agc cct ta... Jβ2.7 Cβ2 | ||||
| 04 | |||||
| Donor | HCMV pp65495-503 | A0201 | 1/1 | Clone 4.1 | Vβ13.1...caa acc ggg aca ggg gtc... Jβ1.2 Cβ1 |
| Recipient | HCMV pp65495-503 | A0201 | 3/3 | Clone 4.2 | Vβ6......ctt gct ctg gga gcc... Jβ2.6 Cβ2 |
| 05 | |||||
| Recipient | HCMV pp65495-503 | A0201 | 2/2 (before alloSCT) | Clone 5.1 | Vβ15.1...g gga cta gcg ggg gct tgg gc... Jβ2.6 Cβ2 |
| Recipient | HCMV pp65495-503 | A0201 | 4/4 | Clone 5.2 | Vβ2.1....t cac ctt ctc... Jβ1.2 Cβ1 |
| 06 | |||||
| Donor | HCMV pp65495-503 | A0201 | \(\left\{\begin{array}{l}1/2\\1/2\end{array}\right\}\) | Clone 6.1a | Vβ15.1...ccc ggg act agc ggg gcc ttt agg... Jβ2.5 Cβ2 |
| Clone 6.1b | Vβ15.1...gac ccc tta cta gcc gac tat... Jβ2.3 Cβ2 | ||||
| Recipient | EBV EBNA3C281-290 | B4402 | 2/2 | Clone 6.2 | Vβ9.1....a ggc ggg cag ggc ggg g... Jβ1.5 Cβ1 |
| 07 | |||||
| Donor | HCMV pp65417-426 | B0702 | 5/5 | Clone 7.1 | Vβ14.1...ggg ccc aat ccg acc c... Jβ1.2 Cβ1 |
| Donor | EBV EBNA3C880-891 | B0702 | 2/2 | Clone 7.2 | Vβ14.1...cag ggg gcg aa... Jβ2.7 Cβ2 |
| 08 | |||||
| Donor | HCMV pp65495-503 | A0201 | 2/2 | Clone 8.1 | Vβ13.1...ccc aca acc ggg aca ggg c... Jβ1.2 Cβ1 |
| 09 | |||||
| Recipient | EBV EBNA3C880-891 | B0702 | 2/2 | Clone 9.1 | Vβ7.2/3...t ctt ggg act agt cac g... Jβ2.1 Cβ2 |
| 10 | |||||
| Donor | HCMV pp65495-503 | A0201 | 1/1 | Clone 10.1 | Vβ13.1...agc cag acc ggg aca ggg t... Jβ1.2 Cβ1 |
| HCMV pp65397-411 | B35 | 2/2 | Clone 10.2 | Vβ13.3...att gga gcg a... Jβ1.2 Cβ2 |
Study no. . | Viral peptide . | HLA restriction . | Number of clones with the same TCR Vβ sequence . | T-cell receptor . | β chain hypervariable nucleotide sequences of virus-specific CD8+ T-cell clones . |
|---|---|---|---|---|---|
| 01 | |||||
| Donor | HCMV pp65495-503 | A0201 | 2/2 | Clone 1.1 | Vβ14.1...gag ggg t... Jβ1.1 Cβ1 |
| Recipient | HCMV pp65495-503 | A0201 | 3/3 | Clone 1.3 | Vβ14.1...gag ggg t... Jβ1.1 Cβ1 |
| Donor | HCMV IE72198-207 | B0801 | 1/1 | Clone 1.2 | Vβ13.1...ggc ccc cta ccc ggg g... Jβ2.1 Cβ1 |
| Recipient | HCMV IE72198-207 | B0801 | 2/2 | Clone 1.4 | Vβ14.1...cc cag aga cgg gat t... Jβ1.1 Cβ1 |
| 02 | |||||
| Donor | HCMV pp65417-426 | B0702 | 3/3 | Clone 2.1 | Vβ6.4....t cgg ggg gag ggc cga ggg g... Jβ2.1 Cβ2 |
| Recipient | HCMV pp65417-426 | B0702 | 2/2 | Clone 2.2 | Vβ6.4....t cgg ggg gag ggc cga ggg g... Jβ2.1 Cβ2 |
| Recipient | EBV EBNA3C880-891 | B0702 | 2/2 | Clone 2.3 | Vβ14.1...c att cag gga g... Jβ1.2 Cβ1 |
| 03 | |||||
| Donor | HCMV pp65495-503 | A0201 | \(\left\{\begin{array}{l}3/4\\1/4\end{array}\right\}\) | Clone 3.1a | Vβ14.1...c cca ggc gcc ccg ggt... Jβ1.6 Cβ1 |
| Clone 3.1b | Vβ14.1...acg gcc ggg agc cct ta... Jβ2.7 Cβ2 | ||||
| Recipient | HCMV pp65495-503 | A0201 | \(\left\{\begin{array}{l}6/8\\2/8\end{array}\right\}\) | Clone 3.2a | Vβ14.1...c cca ggc gcc ccg ggt... Jβ1.6 Cβ1 |
| Clone 3.2b | Vβ14.1...acg gcc ggg agc cct ta... Jβ2.7 Cβ2 | ||||
| 04 | |||||
| Donor | HCMV pp65495-503 | A0201 | 1/1 | Clone 4.1 | Vβ13.1...caa acc ggg aca ggg gtc... Jβ1.2 Cβ1 |
| Recipient | HCMV pp65495-503 | A0201 | 3/3 | Clone 4.2 | Vβ6......ctt gct ctg gga gcc... Jβ2.6 Cβ2 |
| 05 | |||||
| Recipient | HCMV pp65495-503 | A0201 | 2/2 (before alloSCT) | Clone 5.1 | Vβ15.1...g gga cta gcg ggg gct tgg gc... Jβ2.6 Cβ2 |
| Recipient | HCMV pp65495-503 | A0201 | 4/4 | Clone 5.2 | Vβ2.1....t cac ctt ctc... Jβ1.2 Cβ1 |
| 06 | |||||
| Donor | HCMV pp65495-503 | A0201 | \(\left\{\begin{array}{l}1/2\\1/2\end{array}\right\}\) | Clone 6.1a | Vβ15.1...ccc ggg act agc ggg gcc ttt agg... Jβ2.5 Cβ2 |
| Clone 6.1b | Vβ15.1...gac ccc tta cta gcc gac tat... Jβ2.3 Cβ2 | ||||
| Recipient | EBV EBNA3C281-290 | B4402 | 2/2 | Clone 6.2 | Vβ9.1....a ggc ggg cag ggc ggg g... Jβ1.5 Cβ1 |
| 07 | |||||
| Donor | HCMV pp65417-426 | B0702 | 5/5 | Clone 7.1 | Vβ14.1...ggg ccc aat ccg acc c... Jβ1.2 Cβ1 |
| Donor | EBV EBNA3C880-891 | B0702 | 2/2 | Clone 7.2 | Vβ14.1...cag ggg gcg aa... Jβ2.7 Cβ2 |
| 08 | |||||
| Donor | HCMV pp65495-503 | A0201 | 2/2 | Clone 8.1 | Vβ13.1...ccc aca acc ggg aca ggg c... Jβ1.2 Cβ1 |
| 09 | |||||
| Recipient | EBV EBNA3C880-891 | B0702 | 2/2 | Clone 9.1 | Vβ7.2/3...t ctt ggg act agt cac g... Jβ2.1 Cβ2 |
| 10 | |||||
| Donor | HCMV pp65495-503 | A0201 | 1/1 | Clone 10.1 | Vβ13.1...agc cag acc ggg aca ggg t... Jβ1.2 Cβ1 |
| HCMV pp65397-411 | B35 | 2/2 | Clone 10.2 | Vβ13.3...att gga gcg a... Jβ1.2 Cβ2 |
All recipient peptide-specific CD8+ T-cell clones were obtained following alloSCT except for clone 5.1 from recipient 05, which was obtained prior to alloSCT. Donor clones 1.1, 2.1, 3.1a, and 3.1b had identical TCR Vβ chain nucleotide sequences to after alloSC transplant recipient clones 1.3, 2.2, 3.2a, and 3.2b, respectively. No virus-specific donor clones were obtained from donor 05 (HCMV seronegative) or donor 09 (fresh PBMCs not obtainable). Brackets indicate from which donor the clones were derived.