Quantitation of AID+ fraction of high AID-expressing samples
. | Cells/well . | . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample . | 50 . | 100 . | 200 . | 400 . | 1600 . | AID+ fraction, % . | QPCR value* . | ||||
| CLL332 | 5/95 | 15/94 | 36/94 | 51/93 | — | 0.210 | 147 | ||||
| CLL105 | — | 0/32 | — | 3/32 | 0.012 | 32 | |||||
| CLL266 | — | 3/32 | — | 10/32 | 0.062 | 125 | |||||
| CLL357 | — | 1/30 | — | 4/32 | 0.024 | 26 | |||||
. | Cells/well . | . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample . | 50 . | 100 . | 200 . | 400 . | 1600 . | AID+ fraction, % . | QPCR value* . | ||||
| CLL332 | 5/95 | 15/94 | 36/94 | 51/93 | — | 0.210 | 147 | ||||
| CLL105 | — | 0/32 | — | 3/32 | 0.012 | 32 | |||||
| CLL266 | — | 3/32 | — | 10/32 | 0.062 | 125 | |||||
| CLL357 | — | 1/30 | — | 4/32 | 0.024 | 26 | |||||
Summary of the results of the limiting dilution experiments performed on 8 CLL cases and 2 elderly healthy individuals (Figure 4A-B). The results are shown as the ratio of the AID+ wells to VH-positive wells at indicated dilutions for each sample. In the last 2 columns on the right, the AID+ fractions are expressed as a percentage, and the real-time quantitative PCR values are reported for each sample, respectively. — indicates that no experiment was performed at those conditions.
Expression units, as per Figure 2.