9.5-dpc YS and P-Sp EC cocultures support the generation of CFU-S8
Cell groups . | CFU-S . | Cocultures with growth factors CFU-S . | Cocultures without growth factors CFU-S . |
|---|---|---|---|
| Fresh BM Sca-1+ ckit+lin− cells | 10.0 ± 1.2 | — | — |
| Fresh YS ECs | 0 ± 0 | — | — |
| Fresh P-Sp ECs | 0 ± 0 | — | — |
| EC-free | — | 6.0 ± 0.8 | 0 ± 0 |
| 9.5-dpc YS ECs | — | 18.7 ± 2.0† | 3.0 ± 1.0*† |
| 9.5-dpc P-Sp ECs | — | 26.0 ± 2.6† | 4.0 ± 1.0*† |
Cell groups . | CFU-S . | Cocultures with growth factors CFU-S . | Cocultures without growth factors CFU-S . |
|---|---|---|---|
| Fresh BM Sca-1+ ckit+lin− cells | 10.0 ± 1.2 | — | — |
| Fresh YS ECs | 0 ± 0 | — | — |
| Fresh P-Sp ECs | 0 ± 0 | — | — |
| EC-free | — | 6.0 ± 0.8 | 0 ± 0 |
| 9.5-dpc YS ECs | — | 18.7 ± 2.0† | 3.0 ± 1.0*† |
| 9.5-dpc P-Sp ECs | — | 26.0 ± 2.6† | 4.0 ± 1.0*† |
One thousand bone marrow Sca-1+c-kit+lin− cells were cocultured with 10 000 ECs derived from 9.5-dpc YS and P-Sp. Growth factors included 50 ng/mL SCF, 50 ng/mL TPO, and 10 ng/mL IL-6. The total number of CFU-S8 indicates the mean ± SD of 3 to 6 mice in 3 independent experiments.—indicates no data.
P <.05 YS and P-Sp EC cocultures compared with EC-free.
P <.01 YS and P-Sp EC cocultures compared with EC-free and sorted BM cells.