The 9.5-dpc YS and P-Sp EC cocultures support the expansion of marrow CFCs
Cell groups . | CFU-GM . | BFU-E . | CFU-Mix . | Total . |
|---|---|---|---|---|
| With growth factors | ||||
| Fresh Sca-1+ ckit+lin− | (194.0 ± 36.0) | (22.0 ± 6.7) | (20.0 ± 5.4) | (235.0 ± 35.8) |
| EC free | 35.0 ± 7.0 | 8.8 ± 3.1 | 6.9 ± 2.9 | 50.9 ± 9.8 |
| 9.5-dpc YS | 413.0 ± 21.9 | 44.0 ± 11.0 | 30.0 ± 8.4 | 487.0 ± 25.5*† |
| 9.5-dpc P-Sp | 491.0 ± 47.8 | 51.0 ± 15.0 | 39.1 ± 10.8 | 582.0 ± 65.2*† |
| Without growth factors | ||||
| EC free | 0 | 0 | 0 | 0 |
| 9.5-dpc YS | 28.1 ± 6.6 | 4.2 ± 3.5 | 3.0 ± 1.6 | 35.3 ± 6.4 |
| 9.5-dpc P-Sp | 34.9 ± 8.2 | 8.9 ± 4.2 | 4.9 ± 1.4 | 48.7 ± 7.5 |
Cell groups . | CFU-GM . | BFU-E . | CFU-Mix . | Total . |
|---|---|---|---|---|
| With growth factors | ||||
| Fresh Sca-1+ ckit+lin− | (194.0 ± 36.0) | (22.0 ± 6.7) | (20.0 ± 5.4) | (235.0 ± 35.8) |
| EC free | 35.0 ± 7.0 | 8.8 ± 3.1 | 6.9 ± 2.9 | 50.9 ± 9.8 |
| 9.5-dpc YS | 413.0 ± 21.9 | 44.0 ± 11.0 | 30.0 ± 8.4 | 487.0 ± 25.5*† |
| 9.5-dpc P-Sp | 491.0 ± 47.8 | 51.0 ± 15.0 | 39.1 ± 10.8 | 582.0 ± 65.2*† |
| Without growth factors | ||||
| EC free | 0 | 0 | 0 | 0 |
| 9.5-dpc YS | 28.1 ± 6.6 | 4.2 ± 3.5 | 3.0 ± 1.6 | 35.3 ± 6.4 |
| 9.5-dpc P-Sp | 34.9 ± 8.2 | 8.9 ± 4.2 | 4.9 ± 1.4 | 48.7 ± 7.5 |
One thousand BM Sca-1+c-kit+lin− cells were cocultured with 10 000 ECs derived from 9.5-dpc YS and P-Sp. The CFC counts derived from freshly isolated and plated sorted cells reflect the input counts and are enclosed in parentheses. Growth factors used included 50 ng/mL SCF, 50 ng/mL TPO, and 10 ng/mL IL-6. The total number of hematopoietic CFCs indicates the mean ± SD of triplicate cultures of 3 independent experiments.
P <.05 YS and P-Sp coculture cells compared with fresh sorted BM cells.
P <.01 YS and P-Sp coculture cells compared with EC-free cultures.