C-18 blocks fusion of cells expressing internalization-deficient CCR5 and of human macrophages
. | Fusion inhibition with C-18, ID50* . | . | |
|---|---|---|---|
| Target cell . | JR-FL Env,†μg/mL . | BaL Env,†μg/mL . | |
| CEM WT-CCR5.EGFP | 10.5 | 6.3 | |
| CEM M7-CCR5.EGFP | 8.7 | 5.5 | |
| PM1 | 5.8 | 1.7 | |
| Macrophages | 2.4 | 2.2 | |
. | Fusion inhibition with C-18, ID50* . | . | |
|---|---|---|---|
| Target cell . | JR-FL Env,†μg/mL . | BaL Env,†μg/mL . | |
| CEM WT-CCR5.EGFP | 10.5 | 6.3 | |
| CEM M7-CCR5.EGFP | 8.7 | 5.5 | |
| PM1 | 5.8 | 1.7 | |
| Macrophages | 2.4 | 2.2 | |
C-18 (at 3-fold serial dilutions) was preincubated with target cells for one hour at 37°C before mixing (at 1:1 ratio) with effector cells. Syncytia were scored at 3 hours for PM1 cells and at 20 hours for macrophages. The data are representative of 2 experiments performed.
12E1 cells were infected overnight with either vCB28 (JR-FL Env) of vCB43 (Ba-L Env) at 10 pfu/cell.