PM1 cells were incubated either with a mixture of RANTES and MIP-1β (1 μg/mL each) or with C-18 (10 μg/mL) for 2 hours at 37°C. The cells were then washed with staining buffer and were stained with FITC-conjugated 2D7 (anti-CCR5; Pharmingen) or FITC-conjugated Leu 3a (anti-hCD4; Becton Dickinson), or FITC-isotype control in the cold, and were analyzed by flow cytometry. Similar results were obtained after 18 hours of incubation of cells with C-18.

Δ MFC values were calculated by subtracting the mean fluorescent channel (MFC) value of the isotype control from specific antibody staining.

Similar results were obtained with the cell line CEM. NRK. CCR5