T gondii cyclophilin but not human or P falciparum cyclophilin inhibits HIV-1 fusion
Effector/target . | Inhibitor (μg/mL)* . | No. of syncytia . | Inhibition, % . |
|---|---|---|---|
| 12E1 (JR-FL)/PM1 | None | 534 ± 50 | — |
| C-18 (10) | 137 ± 74 | 74 | |
| Human cyclophilin (10) | 548 ± 51 | 0 | |
| P falciparum cyclophilin (10) | 552 ± 40 | 0 |
Effector/target . | Inhibitor (μg/mL)* . | No. of syncytia . | Inhibition, % . |
|---|---|---|---|
| 12E1 (JR-FL)/PM1 | None | 534 ± 50 | — |
| C-18 (10) | 137 ± 74 | 74 | |
| Human cyclophilin (10) | 548 ± 51 | 0 | |
| P falciparum cyclophilin (10) | 552 ± 40 | 0 |
— indicates no inhibition.
T gondii cyclophilin (C-18), human cyclophilin, or P falciparum cyclophilin was added to the PM1 target cells for one hour at 37°C before addition of 12E1 cells expressing JR-FL envelope. Syncytia were scored after 3 hours. Representative of 3 experiments performed.