Table 2.

Summary of one-step RT-PCR and S1 nuclease protection assays performed on In2 bone marrow and fetal liver c-Kit+/CD31high/Ly-6C cells and human CD34+ cells



Single-cell RT-PCR analysis

S1 assay

βmaj/βmin
huβ/huδ
huγ
βH1
huβ
Ln2 bone marrow c-Kit+/CD31high/Ly-6C  18/25   13/26   15/24   3/25   ND  
Ln2 fetal liver c-Kit+/CD31high/Ly-6C  ND   ND   13/26   3/25   ND  
Human bone marrow   —   ND   ND   —   100  
Human CD34+
 

 
17/25
 
16/26
 

 
< 1
 


Single-cell RT-PCR analysis

S1 assay

βmaj/βmin
huβ/huδ
huγ
βH1
huβ
Ln2 bone marrow c-Kit+/CD31high/Ly-6C  18/25   13/26   15/24   3/25   ND  
Ln2 fetal liver c-Kit+/CD31high/Ly-6C  ND   ND   13/26   3/25   ND  
Human bone marrow   —   ND   ND   —   100  
Human CD34+
 

 
17/25
 
16/26
 

 
< 1
 

The level of human β-globin gene expression (determined by S1 nuclease protection assay) in CD34+ cells is relative to the expression level in human bone marrow cells.

ND indicates not done;—, not applicable.