Summary of one-step RT-PCR and S1 nuclease protection assays performed on In2 bone marrow and fetal liver c-Kit+/CD31high/Ly-6C— cells and human CD34+ cells
. | Single-cell RT-PCR analysis . | . | . | . | S1 assay . | |||
|---|---|---|---|---|---|---|---|---|
. | βmaj/βmin . | huβ/huδ . | huγ . | βH1 . | huβ . | |||
| Ln2 bone marrow c-Kit+/CD31high/Ly-6C— | 18/25 | 13/26 | 15/24 | 3/25 | ND | |||
| Ln2 fetal liver c-Kit+/CD31high/Ly-6C— | ND | ND | 13/26 | 3/25 | ND | |||
| Human bone marrow | — | ND | ND | — | 100 | |||
| Human CD34+ | — | 17/25 | 16/26 | — | < 1 | |||
. | Single-cell RT-PCR analysis . | . | . | . | S1 assay . | |||
|---|---|---|---|---|---|---|---|---|
. | βmaj/βmin . | huβ/huδ . | huγ . | βH1 . | huβ . | |||
| Ln2 bone marrow c-Kit+/CD31high/Ly-6C— | 18/25 | 13/26 | 15/24 | 3/25 | ND | |||
| Ln2 fetal liver c-Kit+/CD31high/Ly-6C— | ND | ND | 13/26 | 3/25 | ND | |||
| Human bone marrow | — | ND | ND | — | 100 | |||
| Human CD34+ | — | 17/25 | 16/26 | — | < 1 | |||
The level of human β-globin gene expression (determined by S1 nuclease protection assay) in CD34+ cells is relative to the expression level in human bone marrow cells.
ND indicates not done;—, not applicable.