Cell cycle analysis of erythroblasts expressing different H-ras proteins
Infected bicistronic retrovirus construct . | G1 phase, % . | S phase, % . | G2/M phase, % . |
|---|---|---|---|
| IRES-GFP | 96.0 ± 3.3 | 0.9 ± 0.8 | 3.2 ± 2.5 |
| H-ras.WT-IRES-GFP | 76.2 ± 12.9 | 19.8 ± 13.7 | 4.0 ± 3.4 |
| H-ras.N17-IRES-GFP | 98.7 ± 2.3 | 0.4 ± 0.7 | 0.9 ± 1.6 |
| H-ras.V12-IRES-GFP | 50.9 ± 8.7 | 49.1 ± 8.7 | 0 ± 0 |
Infected bicistronic retrovirus construct . | G1 phase, % . | S phase, % . | G2/M phase, % . |
|---|---|---|---|
| IRES-GFP | 96.0 ± 3.3 | 0.9 ± 0.8 | 3.2 ± 2.5 |
| H-ras.WT-IRES-GFP | 76.2 ± 12.9 | 19.8 ± 13.7 | 4.0 ± 3.4 |
| H-ras.N17-IRES-GFP | 98.7 ± 2.3 | 0.4 ± 0.7 | 0.9 ± 1.6 |
| H-ras.V12-IRES-GFP | 50.9 ± 8.7 | 49.1 ± 8.7 | 0 ± 0 |
TER-119— erythroblasts were purified and infected with different bicistronic retrovirus constructs. Infected erythroblasts were cultured for 2 days in vitro, with Epo on day 1 and without Epo on day 2. Results of infected cells with similar expression level of GFP are presented here as the percentage of total cells analyzed.