Quantification of CFU-E colonies formed by erythroblasts expressing different H-ras mutants
Infected bicistronic retrovirus construct . | CFU-E colonies, % . |
|---|---|
| IRES-hCD4 | 100 |
| H-ras.WT-IRES-hCD4 | 118.0 ± 26.0 |
| H-ras.N17-IRES-hCD4 | 98.3 ± 9.9 |
| H-ras.V12-IRES-hCD4 | 58.2 ± 10.3 |
Infected bicistronic retrovirus construct . | CFU-E colonies, % . |
|---|---|
| IRES-hCD4 | 100 |
| H-ras.WT-IRES-hCD4 | 118.0 ± 26.0 |
| H-ras.N17-IRES-hCD4 | 98.3 ± 9.9 |
| H-ras.V12-IRES-hCD4 | 58.2 ± 10.3 |
Total fetal liver cells were freshly isolated and infected with different bicistronic retrovirus constructs. Results are presented as the percentage of CFU-E colonies formed by fetal liver cells infected with control vector.