The effect of heparin and ionic strength on stoichiometries of thrombin inhibition
. | . | Ionic strength . | . | . | ||
|---|---|---|---|---|---|---|
| Preincubation . | . | 0.15 . | 0.05 . | 0.01 . | ||
| Plasma α | Thrombin | |||||
| HAP | — | 1.1 ± 0.1 | 1.1 ± 0.1 | 1.2 ± 0.1 | ||
| HAP | 4mer | 1.1 ± 0.1 | 1.3 ± 0.1 | 1.5 ± 0.1 | ||
| HAP | 14mer | 1.1 ± 0.1 | 1.3 ± 0.1 | 1.6 ± 0.1 | ||
| — | 4mer | 1.2 ± 0.1 | 1.5 ± 0.2 | 1.8 ± 0.2 | ||
| — | 14mer | 1.2 ± 0.1 | 1.5 ± 0.2 | 1.7 ± 0.2 | ||
| S137A | Thrombin | |||||
| HAP | — | 1.2 ± 0.1 | 1.3 ± 0.1 | 1.5 ± 0.1 | ||
| HAP | 4mer | 1.3 ± 0.1 | 1.5 ± 0.2 | 1.7 ± 0.1 | ||
| HAP | 14mer | 1.3 ± 0.1 | 1.8 ± 0.2 | 2.2 ± 0.1 | ||
| — | 4mer | 1.4 ± 0.1 | 2.1 ± 0.1 | 3.1 ± 0.3 | ||
| — | 14mer | 1.4 ± 0.1 | 3.0 ± 0.3 | 3.6 ± 0.1 | ||
| A384S | Thrombin | |||||
| HAP | — | 1.3 ± 0.2 | 1.4 ± 0.1 | 1.8 ± 0.2 | ||
| HAP | 4mer | 1.5 ± 0.1 | 1.8 ± 0.2 | 2.2 ± 0.2 | ||
| HAP | 14mer | 1.5 ± 0.2 | 2.0 ± 0.1 | 2.8 ± 0.3 | ||
| — | 4mer | 2.2 ± 0.1 | 3.9 ± 0.3 | 9.5 ± 0.2 | ||
| — | 14mer | 2.5 ± 0.2 | 4.5 ± 0.2 | 6.8 ± 0.3 | ||
. | . | Ionic strength . | . | . | ||
|---|---|---|---|---|---|---|
| Preincubation . | . | 0.15 . | 0.05 . | 0.01 . | ||
| Plasma α | Thrombin | |||||
| HAP | — | 1.1 ± 0.1 | 1.1 ± 0.1 | 1.2 ± 0.1 | ||
| HAP | 4mer | 1.1 ± 0.1 | 1.3 ± 0.1 | 1.5 ± 0.1 | ||
| HAP | 14mer | 1.1 ± 0.1 | 1.3 ± 0.1 | 1.6 ± 0.1 | ||
| — | 4mer | 1.2 ± 0.1 | 1.5 ± 0.2 | 1.8 ± 0.2 | ||
| — | 14mer | 1.2 ± 0.1 | 1.5 ± 0.2 | 1.7 ± 0.2 | ||
| S137A | Thrombin | |||||
| HAP | — | 1.2 ± 0.1 | 1.3 ± 0.1 | 1.5 ± 0.1 | ||
| HAP | 4mer | 1.3 ± 0.1 | 1.5 ± 0.2 | 1.7 ± 0.1 | ||
| HAP | 14mer | 1.3 ± 0.1 | 1.8 ± 0.2 | 2.2 ± 0.1 | ||
| — | 4mer | 1.4 ± 0.1 | 2.1 ± 0.1 | 3.1 ± 0.3 | ||
| — | 14mer | 1.4 ± 0.1 | 3.0 ± 0.3 | 3.6 ± 0.1 | ||
| A384S | Thrombin | |||||
| HAP | — | 1.3 ± 0.2 | 1.4 ± 0.1 | 1.8 ± 0.2 | ||
| HAP | 4mer | 1.5 ± 0.1 | 1.8 ± 0.2 | 2.2 ± 0.2 | ||
| HAP | 14mer | 1.5 ± 0.2 | 2.0 ± 0.1 | 2.8 ± 0.3 | ||
| — | 4mer | 2.2 ± 0.1 | 3.9 ± 0.3 | 9.5 ± 0.2 | ||
| — | 14mer | 2.5 ± 0.2 | 4.5 ± 0.2 | 6.8 ± 0.3 | ||
—indicates no preincubation.