Reduced factor-independent colony formation in vitro by BCR-ABL–transduced SHIP–/– LTC-IC–derived CFCs
. | No. of LTC-IC—derived CFCs detected per 103 Sca-1+lin-GFP+ cells . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| CFC assay conditions . | BFU-E . | CFU-GEMM . | CFU-GM . | Total . | |||
| MIG | |||||||
| 3GF | |||||||
| +/+ | 29 ± 4 | 28 ± 3 | 205 ± 77 | 262 ± 84 | |||
| -/- | 25 ± 2 | 26 ± 4 | 210 ± 60 | 265 ± 76 | |||
| EPO | |||||||
| +/+ | 0 | 0 | 0 | 0 | |||
| -/- | 0 | 0 | 0 | 0 | |||
| No GF | |||||||
| +/+ | 0 | 0 | 0 | 0 | |||
| -/- | 0 | 0 | 0 | 0 | |||
| p210 | |||||||
| 3GF | |||||||
| +/+ | 150 ± 30 | 90 ± 30 | 1545 ± 145 | 1785 ± 205 | |||
| -/- | 115 ± 15 | 40 ± 10 | 1440 ± 150 | 1590 ± 220 | |||
| EPO | |||||||
| +/+ | 135 ± 15 | 60 ± 10 | 980 ± 60 | 1180 ± 110 | |||
| -/- | 38 ± 7 | 20 ± 2 | 720 ± 120 | 780 ± 128 | |||
| No GF | |||||||
| +/+ | 80 ± 30 | 40 ± 10 | 930 ± 90 | 1050 ± 220 | |||
| -/- | 20 ± 4 | 0 | 710 ± 120 | 730 ± 120 | |||
. | No. of LTC-IC—derived CFCs detected per 103 Sca-1+lin-GFP+ cells . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| CFC assay conditions . | BFU-E . | CFU-GEMM . | CFU-GM . | Total . | |||
| MIG | |||||||
| 3GF | |||||||
| +/+ | 29 ± 4 | 28 ± 3 | 205 ± 77 | 262 ± 84 | |||
| -/- | 25 ± 2 | 26 ± 4 | 210 ± 60 | 265 ± 76 | |||
| EPO | |||||||
| +/+ | 0 | 0 | 0 | 0 | |||
| -/- | 0 | 0 | 0 | 0 | |||
| No GF | |||||||
| +/+ | 0 | 0 | 0 | 0 | |||
| -/- | 0 | 0 | 0 | 0 | |||
| p210 | |||||||
| 3GF | |||||||
| +/+ | 150 ± 30 | 90 ± 30 | 1545 ± 145 | 1785 ± 205 | |||
| -/- | 115 ± 15 | 40 ± 10 | 1440 ± 150 | 1590 ± 220 | |||
| EPO | |||||||
| +/+ | 135 ± 15 | 60 ± 10 | 980 ± 60 | 1180 ± 110 | |||
| -/- | 38 ± 7 | 20 ± 2 | 720 ± 120 | 780 ± 128 | |||
| No GF | |||||||
| +/+ | 80 ± 30 | 40 ± 10 | 930 ± 90 | 1050 ± 220 | |||
| -/- | 20 ± 4 | 0 | 710 ± 120 | 730 ± 120 | |||
Each 1-mL LTC-IC assay was initiated with 103 Sca-1+lin-GFP+ cells. The LTCs were harvested after 4 weeks, and the cells in them were then assayed for CFCs in FCS-containing methylcellulose medium in the presence or absence of growth factors. Data shown are the total CFCs recovered per initial LTC and represent mean ± SEM of values obtained from 3 independent experiments.