Table 3. Reduced factor-independent... | American Society of Hematology

Table 3.

Reduced factor-independent colony formation by BCR-ABL–transduced SHIP^–/– CFCs in vitro

	No. of CFCs detected per 10³ Sca-1⁺lin^-GFP⁺ cells
CFC assay conditions	BFU-E	CFU-GEMM	CFU-GM	Total
MIG
3GF
+/+	15 ± 3	12 ± 4	85 ± 10	112 ± 12
-/-	12 ± 3	10 ± 4	95 ± 12	118 ± 14
EPO
+/+	0	0	0	0
-/-	0	0	0	0
No GF
+/+	0	0	0	0
-/-	0	0	0	0
p210
3GF
+/+	50 ± 8	30 ± 6	215 ± 25	295 ± 35
-/-	20 ± 6	35 ± 8	135 ± 20	198 ± 32
EPO
+/+	20 ± 2	23 ± 6	128 ± 12	172 ± 18
-/-	16 ± 4	6 ± 3	82 ± 8	105 ± 4
No GF
+/+	14 ± 2	20 ± 4	90 ± 8	124 ± 12
-/-	8 ± 2	0	68 ± 6	76 ± 6

	No. of CFCs detected per 10³ Sca-1⁺lin^-GFP⁺ cells
CFC assay conditions	BFU-E	CFU-GEMM	CFU-GM	Total
MIG
3GF
+/+	15 ± 3	12 ± 4	85 ± 10	112 ± 12
-/-	12 ± 3	10 ± 4	95 ± 12	118 ± 14
EPO
+/+	0	0	0	0
-/-	0	0	0	0
No GF
+/+	0	0	0	0
-/-	0	0	0	0
p210
3GF
+/+	50 ± 8	30 ± 6	215 ± 25	295 ± 35
-/-	20 ± 6	35 ± 8	135 ± 20	198 ± 32
EPO
+/+	20 ± 2	23 ± 6	128 ± 12	172 ± 18
-/-	16 ± 4	6 ± 3	82 ± 8	105 ± 4
No GF
+/+	14 ± 2	20 ± 4	90 ± 8	124 ± 12
-/-	8 ± 2	0	68 ± 6	76 ± 6

Each 1-mL CFC assay culture contained 10³ Sca-1⁺lin^-GFP⁺ cells. Data shown are the mean ± SEM of values obtained from 3 independent experiments. BFU-E indicates erythroid burst-forming unit; CFU-GEMM, granulocyte-erythroid-macrophage-megakaryocyte, colony-forming unit; CFU-GM, granulocyte-macrophage colony-forming unit; and GF, growth factor.

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