Leukemia-initiating cell frequency (L-IC) of BCR-ABL–transduced Sca-1+lin–GFP+ cells from +/+ and SHIP–/– mice
Phenotype of cells injected per mouse . | No. of leukemic mice/no. of mice receiving transplants . | Maximum WBC count, × 106/mL . |
|---|---|---|
| +/+ Sca-1+lin-GFP+ | ||
| 10 | 0/8 | 6 |
| 100 | 0/8 | 8 |
| 1000 | 8/8 | 26 |
| 3000 | 5/5 | 28 |
| 5000 | 5/5 | 36 |
| 10 000 | 5/5 | 40 |
| 20 000 | 5/5 | 60 |
| SHIP-/- Sca-1+lin-GFP+ | ||
| 10 | 0/8 | 7 |
| 100 | 0/8 | 8 |
| 1000 | 7/7 | 24 |
| 3000 | 6/6 | 27 |
| 5000 | 5/5 | 38 |
| 10 000 | 5/5 | 42 |
| 20 000 | 5/5 | 58 |
Phenotype of cells injected per mouse . | No. of leukemic mice/no. of mice receiving transplants . | Maximum WBC count, × 106/mL . |
|---|---|---|
| +/+ Sca-1+lin-GFP+ | ||
| 10 | 0/8 | 6 |
| 100 | 0/8 | 8 |
| 1000 | 8/8 | 26 |
| 3000 | 5/5 | 28 |
| 5000 | 5/5 | 36 |
| 10 000 | 5/5 | 40 |
| 20 000 | 5/5 | 60 |
| SHIP-/- Sca-1+lin-GFP+ | ||
| 10 | 0/8 | 7 |
| 100 | 0/8 | 8 |
| 1000 | 7/7 | 24 |
| 3000 | 6/6 | 27 |
| 5000 | 5/5 | 38 |
| 10 000 | 5/5 | 42 |
| 20 000 | 5/5 | 58 |
L-IC frequency is 1 in 430 (95% CI, 202-900) for +/+ Sca-1+lin-GFP+ cells and 1 in 450 (95% CI, 207-984) for SHIP-/- Sca-1+lin-GFP+ cells in vivo.