Stem cell frequency (CRU) of MIG-transduced Sca-1+lin–GFP+ BM cells from +/+ and SHIP–/– mice
Phenotype of cells injected per mouse . | No. of mice positive for GFP+ lymphoid-myeloid cells/no. of mice receiving transplants . |
|---|---|
| +/+ Sca-1+lin-GFP+ | |
| 10 | 0/3 |
| 100 | 5/6 |
| 1000 | 6/6 |
| 3000 | 3/3 |
| 5000 | 3/3 |
| 10 000 | 3/3 |
| SHIP-/- Sca-1+lin-GFP+ | |
| 10 | 0/3 |
| 100 | 4/5 |
| 1000 | 5/5 |
| 3000 | 3/3 |
| 5000 | 3/3 |
| 10 000 | 3/3 |
Phenotype of cells injected per mouse . | No. of mice positive for GFP+ lymphoid-myeloid cells/no. of mice receiving transplants . |
|---|---|
| +/+ Sca-1+lin-GFP+ | |
| 10 | 0/3 |
| 100 | 5/6 |
| 1000 | 6/6 |
| 3000 | 3/3 |
| 5000 | 3/3 |
| 10 000 | 3/3 |
| SHIP-/- Sca-1+lin-GFP+ | |
| 10 | 0/3 |
| 100 | 4/5 |
| 1000 | 5/5 |
| 3000 | 3/3 |
| 5000 | 3/3 |
| 10 000 | 3/3 |
Stem cell frequency is 1 in 65 (95% confidence interval [CI], 39-100) for +/+ Sca-1+lin-GFP+ cells and 1 in 70 (95% CI, 42-120) for SHIP-/- Sca-1+lin-GFP+ cells in vivo.